Shandong Second Medical University, Weifang, 261053, China.
Zhucheng Center for Disease Control and Prevention, Weifang, 262200, China.
Acta Trop. 2024 Sep;257:107322. doi: 10.1016/j.actatropica.2024.107322. Epub 2024 Jul 14.
Arboviruses have always been a significant public health concern. Metagenomic surveillance has expanded the number of novel, often unclassified arboviruses, especially mosquito-borne and mosquito-specific viruses. This report presents the first description of a novel single-stranded RNA virus, Wanghe virus, identified from mosquitoes that were collected in Shandong Province in 2022. In this study, a total of 4,795 mosquitoes were collected and then divided into 105 pools according to location and species. QRT-PCR and nested PCR were performed to confirm the presence of Wanghe virus, and its genomic features and phylogenetic relationships were further analyzed. Our results revealed that Wanghe virus was detected in 9 out of the 105 mosquito pools, resulting in a minimum infection rate (MIR) of 0.19 % (9/4,795). One complete genome sequence and three viral partial sequences were obtained from the Wanghe virus-positive pools. Pairwise distance analysis indicated that these amplified sequences shared high nucleotide identity. Phylogenetic analysis demonstrated that Wanghe virus is most closely related to Guiyang Solinvi-like virus 3, which belongs to Solinviviridae. Further analyses indicated that Wanghe virus is a new, unclassified member of Solinviviridae.
虫媒病毒一直是重大公共卫生关切。宏基因组监测扩大了新型、通常未分类的虫媒病毒的数量，尤其是蚊媒和蚊特异性病毒。本报告首次描述了一种新型单链 RNA 病毒，即王河病毒，该病毒从 2022 年在山东省采集的蚊子中鉴定出来。在这项研究中，共收集了 4795 只蚊子，然后根据地点和种类分为 105 个池。进行了 QRT-PCR 和巢式 PCR 以确认王河病毒的存在，并进一步分析了其基因组特征和系统发育关系。我们的结果显示，在 105 个蚊子池中检测到了 9 个王河病毒，最低感染率（MIR）为 0.19%（9/4795）。从王河病毒阳性池中获得了一个完整基因组序列和三个病毒部分序列。成对距离分析表明，这些扩增序列具有高度核苷酸同一性。系统发育分析表明，王河病毒与归属于 Solinviviridae 的贵阳索林病毒 3 最为密切相关。进一步分析表明，王河病毒是 Solinviviridae 的一种新的、未分类的成员。
