Laboratório de Baculovírus, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília (UnB), Brasília 70910-900, Brazil.
Laboratório de Bioinformática e Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, Brazil.
Viruses. 2024 Aug 9;16(8):1276. doi: 10.3390/v16081276.
Studies on animal virome have mainly concentrated on chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting a major part of mosquito species diversity. Despite their potential role in arbovirus transmission, the viromes of sylvatic mosquitoes remain largely unexplored. These mosquitoes may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation and its status as a biodiversity hotspot, offers an ideal setting for investigating mosquito viromes due to potential zoonotic spillover risks from land use changes. This study aimed to characterize the viromes of sylvatic mosquitoes collected from various locations within Minas Gerais state, Brazil. The total RNA was extracted from mosquito pools of , , , , and species, followed by high-throughput sequencing (HTS). Bioinformatic analysis included quality control, contig assembly, and viral detection. Sequencing data analysis revealed 11 near-complete viral genomes (new viruses are indicated with asterisks) across seven viral families and one unassigned genus. These included: (Ferox mosquito mononega-like virus* and Albipes mosquito Gordis-like virus*), (Sabethes albiprivus phasmavirus*), (Pedras lispivirus variant MG), (Sabethes albiprivus iflavivirus*), (Buriti virga-like virus variant MG and Sabethes albiprivus virgavirus 1*), (Psorophora ferox flavivirus*), (Alphamesonivirus cavallyense variant MG), and the genus (Biggie virus variant MG virus and Coquillettidia venezuelensis negevirus*). Moreover, the presence of ISVs and potential novel arboviruses underscores the need for ongoing surveillance and control strategies to mitigate the risk of emerging infectious diseases.
对动物病毒组的研究主要集中在脊索动物和具有医学意义的无脊椎动物上，往往忽略了构成蚊科物种多样性主要部分的森林蚊子。尽管它们在虫媒病毒传播中具有潜在作用，但森林蚊子的病毒组仍在很大程度上未被探索。这些蚊子也可能携带昆虫特异性病毒（ISV），影响虫媒病毒的传播动态。塞拉多生物群落以快速森林砍伐和生物多样性热点地区而闻名，由于土地利用变化带来的潜在人畜共患病溢出风险，为研究蚊子病毒组提供了理想的环境。本研究旨在描绘从巴西米纳斯吉拉斯州不同地点采集的森林蚊子的病毒组。从 、 、 、 和 蚊种的蚊群中提取总 RNA，然后进行高通量测序（HTS）。生物信息学分析包括质量控制、连续序列组装和病毒检测。测序数据分析显示，在七个病毒科和一个未分类属中发现了 11 个近完整的病毒基因组（新病毒用星号表示）。其中包括： （费罗克斯蚊单囊病毒和阿尔比佩斯蚊戈尔迪斯病毒*）、 （萨贝特斯白纹病毒）、 （佩德拉斯利斯皮病毒变异 MG）、 （萨贝特斯白纹病毒黄病毒*）、 （伯蒂病毒样病毒变异 MG 和萨贝特斯白纹病毒病毒 1*）、 （普索罗弗拉蚊黄病毒*）、 （阿尔法梅索尼病毒卡瓦利恩塞变种 MG）和属 （大比病毒变异 MG 病毒和委内瑞拉库伊莱特蒂亚病毒*）。此外，ISV 和潜在的新型虫媒病毒的存在突显了持续监测和控制策略的必要性，以减轻新发传染病的风险。
