Lu Ke, Wang Yongqin, Wang Yanjun, Zhang Qingqing, Zhu Yujing, Yu Xiaoli, Niu Guoyu
Shandong Second Medical University, Weifang, 261053, China.
Weifang People's Hospital, Shandong Second Medical University, Weifang, 261000, China.
Heliyon. 2025 Feb 7;11(4):e42532. doi: 10.1016/j.heliyon.2025.e42532. eCollection 2025 Feb 28.
Climate change is increasing the threat of arboviruses globally, amplifying their geographic distribution and virulence. This poses a grave risk to public health as mosquito-transmitted viral infections resurge and spread. Unbiased metagenomics plays a crucial role in identifying unknown pathogens and monitoring significant ones. Using this approach, a novel mosquito-associated virus, named Chijiahe virus, was discovered. We collected 2910 mosquitoes and divided them into 65 pools based on species and collection site. To ascertain the existence of Chijiahe virus, we utilized nested PCR alongside real-time quantitative reverse transcription PCR (RT-qPCR). Further analysis of the virus's genomic characteristics and phylogenetic relationships revealed its detection in 10 out of 65 mosquito pools, yielding a minimum infection rate (MIR) of 0.34 %. The virus genome consists of a 9654-nucleotide single-stranded RNA, containing an open reading frame (ORF) that encodes essential proteins, including the capsid protein, RNA helicase, and RNA-dependent RNA polymerase (RdRp). Pairwise distance analysis revealed high nucleotide identity among all obtained sequences. Phylogenetic analysis demonstrated Chijiahe virus's close relationship to Arthropod iflavirus, a member of the family . Additional analyses confirmed Chijiahe virus as a novel member of .
气候变化正在全球范围内增加虫媒病毒的威胁，扩大其地理分布和毒力。随着蚊媒病毒感染的再度流行和传播，这对公众健康构成了严重风险。无偏宏基因组学在识别未知病原体和监测重要病原体方面发挥着关键作用。通过这种方法，发现了一种新的与蚊子相关的病毒，命名为赤家河病毒。我们收集了2910只蚊子，并根据物种和采集地点将它们分成65组。为了确定赤家河病毒的存在，我们使用了巢式PCR以及实时定量逆转录PCR（RT-qPCR）。对该病毒的基因组特征和系统发育关系的进一步分析显示，在65组蚊子中有10组检测到该病毒，最低感染率（MIR）为0.34%。该病毒基因组由一个9654个核苷酸的单链RNA组成，包含一个开放阅读框（ORF），该开放阅读框编码包括衣壳蛋白、RNA解旋酶和RNA依赖性RNA聚合酶（RdRp）在内的必需蛋白。成对距离分析显示，所有获得的序列之间具有高度的核苷酸同一性。系统发育分析表明，赤家河病毒与节肢动物黄病毒属（该科的一个成员）关系密切。进一步分析证实赤家河病毒是该属的一个新成员。
