Chen Qi, Pan Yongsheng, Yang Xiufeng, Zhu Hua, Zheng Bing, Ju Longtao
Department of Surgery (Prostate), Affiliated Nantong Stomatological Hospital of Nantong University (Nantong Integrated Traditional Chinese and Western Medicine Hospital), Nantong, China.
Department of Urology, Affiliated Hospital 2 of Nantong University (Nantong First People's Hospital), Nantong, China.
Nucleosides Nucleotides Nucleic Acids. 2025;44(7):587-597. doi: 10.1080/15257770.2024.2372315. Epub 2024 Jul 14.
Prostate cancer is an adverse tumor that occurs in the male reproductive system. The symptoms of patients in the early stage are not obvious and are generally difficult to detect.
The aim of this study was to determine the regulation of lncRNA GABPB1-AS1 (GABPB1-AS1) on prostate cancer progression and explore the diagnostic potential of GABPB1-AS1.
The contents of serum GABPB1-AS1 and miR-330-3p were examined by RT-qPCR assay. The functions of silencing GABPB1-AS1 and miR-330-3p inhibitor in prostate cancer cells were determined using transfection assay, CCK-8 assay and Transwell assay. The target of GABPB1-AS1 was predicted and verified at the molecular level by bioinformatics and luciferase reporter gene assay. The function of GABPB1-AS1 in prostate cancer diagnosis was evaluated ROC method.
GABPB1-AS1 was upregulated in prostate cancer serum, which was associated with patients' Gleason score and TNM stage. Mechanistically, GABPB1-AS1 directly targeted miR-330-3p, and there was a negative correlation between them. Reduced levels of GABPB1-AS1 in cells after knockdown of GABPB1-AS1 resulted in decreased prostate cancer cell growth and activity, and these inhibitory effects were repaired by miR-330-3p inhibitor.
The present study confirmed that GABPB1-AS1 was overexpressed in prostate cancer, and its sponge miR-330-3p may be an effective target for timely diagnosis of prostate cancer.
前列腺癌是发生于男性生殖系统的一种恶性肿瘤。早期患者症状不明显，一般难以检测到。
本研究旨在确定长链非编码RNA GABPB1-AS1（GABPB1-AS1）对前列腺癌进展的调控作用，并探索GABPB1-AS1的诊断潜力。
采用RT-qPCR法检测血清GABPB1-AS1和miR-330-3p的含量。通过转染实验、CCK-8实验和Transwell实验确定沉默GABPB1-AS1和miR-330-3p抑制剂在前列腺癌细胞中的作用。通过生物信息学和荧光素酶报告基因实验在分子水平预测并验证GABPB1-AS1的靶点。采用ROC法评估GABPB1-AS1在前列腺癌诊断中的作用。
GABPB1-AS1在前列腺癌血清中上调，这与患者的Gleason评分和TNM分期相关。机制上，GABPB1-AS1直接靶向miR-330-3p，二者呈负相关。敲低GABPB1-AS1后细胞中GABPB1-AS1水平降低，导致前列腺癌细胞生长和活性降低，而这些抑制作用可被miR-330-3p抑制剂修复。
本研究证实GABPB1-AS1在前列腺癌中过表达，其作为miR-330-3p的海绵可能是前列腺癌早期诊断的有效靶点。
