Wang Mei, Qiu Xiufeng, Xu Youhong, Zhao Leran, Liu Quanzhong, You Cong
Department of Dermatology, School of Medicine, Tianjin First Central Hospital, Nankai University, Tianjin, 300190, China.
Department of Dermatology and Venereology, The First Affiliated Hospital of Gannan Medical University, No. 23 Qingnian Road, Zhanggong District, Ganzhou, 341000, China.
Arch Microbiol. 2025 May 31;207(7):164. doi: 10.1007/s00203-025-04365-5.
This study investigated the impacts of penicillin G on the Chlamydia trachomatis (CT) Serovar D and host cells in urogenital infections. HeLa cells were infected with CT for 2 h and then treated with different concentrations of penicillin G. The aberrant CT group was compared to untreated CT using dual RNA sequencing, lncRNA sequencing, and bioinformatics analysis. Treatment with 150 U/ml penicillin G for 48 h caused significant abnormalities in CT inclusion bodies. A total of 93 differentially expressed mRNAs were involved in biological processes such as protein modification, cofactor biosynthesis, and response to stimuli. Key pathways affected included peptidoglycan biosynthesis and degradation, oxidative phosphorylation, and bacterial secretion systems. Additionally, penicillin G induced significant changes in CT morphology and mRNA expression, as well as in host HeLa cellular mRNA and lncRNA profiles. These changes may contribute to chronic inflammation, treatment resistance, and potentially tumorigenesis.
本研究调查了青霉素G对泌尿生殖系统感染中沙眼衣原体（CT）血清型D及宿主细胞的影响。用CT感染HeLa细胞2小时，然后用不同浓度的青霉素G进行处理。使用双RNA测序、lncRNA测序和生物信息学分析，将异常CT组与未处理的CT进行比较。用150 U/ml青霉素G处理48小时导致CT包涵体出现显著异常。共有93个差异表达的mRNA参与了蛋白质修饰、辅因子生物合成和对刺激的反应等生物学过程。受影响的关键途径包括肽聚糖生物合成与降解、氧化磷酸化和细菌分泌系统。此外，青霉素G诱导了CT形态和mRNA表达以及宿主HeLa细胞mRNA和lncRNA谱的显著变化。这些变化可能导致慢性炎症、治疗抗性，并可能引发肿瘤发生。
