Easwaran Sreesankar, Montell Denise J
Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106.
bioRxiv. 2024 Dec 11:2024.03.10.584341. doi: 10.1101/2024.03.10.584341.
The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption of development and reproduction upon the return of favorable conditions. Diapause is a complex trait, so we leveraged the genetic reference panel (DGRP) lines and conducted a Genome-Wide Association Study (GWAS) to characterize the genetic basis of diapause. We assessed post-diapause and non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 genetic variants, encompassing single nucleotide polymorphisms, insertions and deletions associated with post-diapause fecundity. We identified 291 candidate diapause-associated genes, 40 of which had previously been associated with diapause, and 89 of which were associated with more than one SNP. Gene network analysis indicated that the diapause-associated genes were primarily linked to neuronal and reproductive system development. Similarly, comparison with results from other fly GWAS revealed the greatest overlap with olfactory-behavior-associated and fecundity-and-lifespan-associated genes. An RNAi screen of selected candidates identified two neuronal genes, Dip- and Scribbler, to be required during recovery for post-diapause fecundity. We complemented the genetic analysis with a test of which neurons are required for successful diapause. We found that although amputation of the antenna had little to no effect on non-diapause lifespan, it reduced diapause lifespan and postdiapause fecundity. We further show that olfactory receptor neurons and temperature-sensing neurons are required for successful recovery from diapause. Our results provide insights into the molecular, cellular, and genetic basis of adult reproductive diapause in .
随着气候变化，环境压力对动物生命的影响日益重要。滞育是一种休眠程序，它会在不利环境下发生，随后在有利条件恢复时恢复发育和繁殖。滞育是一种复杂的性状，因此我们利用遗传参考面板（DGRP）品系并进行了全基因组关联研究（GWAS）来表征滞育的遗传基础。我们评估了193个DGRP品系的滞育后和非滞育繁殖力。GWAS揭示了546个遗传变异，包括与滞育后繁殖力相关的单核苷酸多态性、插入和缺失。我们鉴定出291个候选滞育相关基因，其中40个先前已与滞育相关，89个与多个单核苷酸多态性相关。基因网络分析表明，滞育相关基因主要与神经元和生殖系统发育有关。同样，与其他果蝇GWAS结果的比较显示，与嗅觉行为相关以及繁殖力和寿命相关基因的重叠最大。对选定候选基因的RNAi筛选确定了两个神经元基因Dip和Scribbler，它们在滞育后繁殖力恢复过程中是必需的。我们通过测试成功滞育需要哪些神经元来补充遗传分析。我们发现，虽然切除触角对非滞育寿命几乎没有影响，但它会缩短滞育寿命和滞育后繁殖力。我们进一步表明，嗅觉受体神经元和温度感应神经元是滞育成功恢复所必需的。我们的结果为[具体物种]成年生殖滞育的分子、细胞和遗传基础提供了见解。
