Mardani Rajab, Alavi Ariana, Mousavi Nasab Seyed Dawood, Ahmadi Nayebali, Hossein Tehrani Mohammad Javad, Shahali Maryam, Doroud Delaram
Department of Viral Vaccines, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran.
Department of Production, Bsc in Biotechnology Pharmaceutical and Drug Delivery System Labratoary, Pasteur Institute of Iran, Tehren, Iran.
Iran J Microbiol. 2024 Jun;16(3):351-356. doi: 10.18502/ijm.v16i3.15767.
Lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. TNF-α and IL-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection.
In this study, mannan was extracted and processed, and then Bulb/c female mice were used in three groups, one group was given BCG vaccine, the other group was given BCG vaccine with mannan adjuvant, and a non-injected group was used as a control group. Inflammatory factors interleukin-12, TNF-α, IgG and IgM were measured in mouse serum.
The levels of the inflammatory factors interleukin-12 and TNF-α in the serum isolated from mice receiving the BCG vaccine with mannan adjuvant showed a significant difference compared to the group that received only the BCG vaccine and the control group [IL-12] and , with P≤0.05.The examination of the level of IgG immune factors in these three groups revealed a significant difference. The group that received the BCG vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the BCG vaccine and the control group, with P≤0.05. The level of IgM was higher in the group that received the BCG vaccine alone compared to the adjuvant vaccine group and the control group, with P≤0.05.
Our results indicated that mice receiving the BCG vaccine with mannan adjuvant had significantly higher serum levels of IL-12, TNF-α, and IgG than the group receiving BCG alone.
脂阿拉伯甘露聚糖是分枝杆菌重要结构细胞表面的成分之一，作为一种免疫刺激因子。肿瘤坏死因子-α（TNF-α）和白细胞介素-12（IL-12）是感染期间被激活和诱导的抗菌炎性细胞因子的两个例子。
在本研究中，提取并处理了甘露聚糖，然后将雌性Balb/c小鼠分为三组，一组给予卡介苗疫苗，另一组给予含甘露聚糖佐剂的卡介苗疫苗，未注射组作为对照组。检测小鼠血清中的炎性因子白细胞介素-12、肿瘤坏死因子-α、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）。
与仅接受卡介苗疫苗的组和对照组相比，接受含甘露聚糖佐剂的卡介苗疫苗的小鼠血清中炎性因子白细胞介素-12和肿瘤坏死因子-α的水平显示出显著差异[IL-12] ，P≤0.05。对这三组IgG免疫因子水平的检测显示出显著差异。与仅接受卡介苗疫苗的组和对照组相比，接受含甘露聚糖佐剂的卡介苗疫苗的组显示出明显差异，P≤0.05。与佐剂疫苗组和对照组相比，仅接受卡介苗疫苗的组中IgM水平更高，P≤0.05。
我们的结果表明，接受含甘露聚糖佐剂的卡介苗疫苗的小鼠血清中IL-12、TNF-α和IgG的水平显著高于仅接受卡介苗的组。
