Mojarrad Saber, Tavakoli Movaghar Nahid, Edalat Fahime, Letafati Arash, Kargar Jahromi Zahra, Moattari Afagh
Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2024 Jun;16(3):411-420. doi: 10.18502/ijm.v16i3.15798.
The pediatric population worldwide bears a significant morbidity and death burden due to acute respiratory infections (ARIs). Human Orthopneumovirus, sometimes referred to as the Human Respiratory Syncytial Virus (HRSV), is one of the main causes of ARIs in infants. The main goal of this study was to identify the genetic diversity of HRSV strains that were circulating in the Iranian population at a certain time of year.
Two hundred youngsters less than 12 years old with acute respiratory infections had samples taken from their throat and pharynx secretions. Then, external and hemi-nested PCR were employed, using specific primers targeting the G gene region to detect HRSV. Subsequently, nine randomly selected positive samples were subjected to sequencing. The results were then compared with reference strains cataloged in GeneBank, and phylogenetic tree was constructed using Chromes and MEGA7.
Out of 200 samples, 34 were identified as containing HRSV. Subgroup A was predominant, accounting for 61.76% of cases, followed by subgroup BA (35.29%) and subgroup B (2.94%). Phylogenetic analysis revealed five samples associated with subtype B and four with genotype A. Genomic analysis showed three samples under the GA2 subgroup and one under GA1 for subtype A, and four samples in subgroup BA and one in GB2 for subtype B.
In this study, subgroup A strains, particularly genotype GA2, exhibited a higher prevalence compared to subgroup B strains during the specific period under investigation, shedding light on the genetic landscape of HRSV in this region.
全球儿童群体因急性呼吸道感染（ARI）承受着巨大的发病和死亡负担。人正肺病毒，有时被称为人呼吸道合胞病毒（HRSV），是婴儿ARI的主要病因之一。本研究的主要目标是确定在一年中特定时间段内在伊朗人群中传播的HRSV毒株的遗传多样性。
从200名12岁以下患有急性呼吸道感染的儿童的咽喉分泌物中采集样本。然后，使用靶向G基因区域的特异性引物，采用外部和半巢式PCR来检测HRSV。随后，对9个随机选择的阳性样本进行测序。然后将结果与基因库中编目的参考毒株进行比较，并使用Chromes和MEGA7构建系统发育树。
在200个样本中，有34个被鉴定为含有HRSV。A亚组占主导，占病例的61.76%，其次是BA亚组（35.29%）和B亚组（2.94%）。系统发育分析显示，有5个样本与B亚型相关，4个与A基因型相关。基因组分析显示，A亚型中有3个样本属于GA2亚组，1个属于GA1亚组；B亚型中有4个样本属于BA亚组，1个属于GB2亚组。
在本研究中，在调查的特定时期内，A亚组毒株，特别是GA2基因型，比B亚组毒株表现出更高的流行率，这揭示了该地区HRSV的遗传格局。
