Ghiasi Seyyed Ehsan, Gheibipour Maryam, Motamedi Hossein, Dar Mudasir A
Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran.
Research Group of Environmental Stress in Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran.
Iran J Microbiol. 2024 Jun;16(3):389-400. doi: 10.18502/ijm.v16i3.15796.
Rumen microbiologists are looking for new probiotics to improve the digestibility of livestock diets. This study intended to screen and evaluate the ruminal cellulolytic bacteria (CBs) and their potential application as probiotics.
Microbial culture and molecular techniques performed to isolate CBs from the rumen of camels, deer and rams. Their antibacterial and antibiogram tests were done using disc diffusion method. Their potential to degrade cellulose, starch, tannin and protein were investigated using clear zone halo, and spectrophotometric techniques. Bilious, saline, and acidic broth media were used to study the resistance of isolates in intestinal conditions.
The phylogenetic analysis revealed that the strains belonged to Firmicutes and Proteobacteria phyla, and . The highest cellulase (CAS) activity was recorded by Dez wildlife13A (2.98 UmL), whereas Loot desert 111A (1.14 Uml) was produced the lowest enzyme. The isolates were highly resistant to synthetic conditions of intestine (pH 2.5-3.5, bile 0.3-2%), as well as tolerated higher concentrations of NaCl (up to 10%). They effectively inhibited standard pathogen strains, and showed sensitivity to the used antibiotics.
This study reports the cellulolytic Tabbas desert 32A for the first time from the rumen, which will have potential biotechnological applications.
瘤胃微生物学家正在寻找新的益生菌，以提高家畜日粮的消化率。本研究旨在筛选和评估瘤胃纤维素分解菌（CBs）及其作为益生菌的潜在应用。
采用微生物培养和分子技术从骆驼、鹿和公羊的瘤胃中分离CBs。使用纸片扩散法进行它们的抗菌和药敏试验。使用透明圈晕圈法和分光光度技术研究它们降解纤维素、淀粉、单宁和蛋白质的潜力。使用胆汁、生理盐水和酸性肉汤培养基研究分离株在肠道条件下的抗性。
系统发育分析表明，这些菌株属于厚壁菌门和变形菌门。Dez wildlife13A记录的纤维素酶（CAS）活性最高（2.98 U/mL），而Loot desert 111A产生的酶最低（1.14 U/mL）。分离株对肠道的模拟条件（pH 2.5 - 3.5，胆汁0.3 - 2%）具有高度抗性，并且能耐受较高浓度的NaCl（高达10%）。它们有效抑制标准病原菌菌株，并且对所用抗生素敏感。
本研究首次报道了来自瘤胃的纤维素分解菌Tabbas desert 32A，其将具有潜在的生物技术应用。
