Chang Wuguang, Gao Wuyou, Liu Die, Luo Bin, Li Hongmu, Zhong Leqi, Chen Youfang
Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University Zhuhai 519000, Guangdong, P. R. China.
Department of Thoracic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Guangzhou 510060, Guangdong, P. R. China.
Am J Cancer Res. 2024 Jun 15;14(6):2823-2838. doi: 10.62347/OBES4130. eCollection 2024.
Tissue transglutaminase (TGM2) is a member of the glutamine transferase superfamily, located within cells and their membranes. When secreted, it catalyzes the cross-linking of extracellular matrix proteins and promotes the formation of extracellular matrix scaffolds. To determine the function of TGM2 in the tumorigenesis of lung squamous cell carcinoma (LUSC), we conducted a comprehensive bioinformatics analysis of TGM2. Our findings indicate that high expression of TGM2 in LUSC was associated with a poorer prognosis. Additionally, we found that high expression of TGM2 is closely related to tumor-promoting inflammation and may increase sensitivity to immunotherapy. We further confirmed the cancer-promoting effect of TGM2 in LUSC through in vitro overexpression and knockdown experiments and showed that TGM2 primarily affects cancer cell proliferation, apoptosis, and invasion. In summary, TGM2 promoted the progression of LUSC, and targeting TGM2 is expected to become a new therapeutic approach for LUSC treatment.
组织转谷氨酰胺酶（TGM2）是谷氨酰胺转移酶超家族的成员，位于细胞及其细胞膜内。分泌时，它催化细胞外基质蛋白的交联，并促进细胞外基质支架的形成。为了确定TGM2在肺鳞状细胞癌（LUSC）肿瘤发生中的作用，我们对TGM2进行了全面的生物信息学分析。我们的研究结果表明，TGM2在LUSC中的高表达与较差的预后相关。此外，我们发现TGM2的高表达与促肿瘤炎症密切相关，并且可能增加对免疫疗法的敏感性。我们通过体外过表达和敲低实验进一步证实了TGM2在LUSC中的促癌作用，并表明TGM2主要影响癌细胞的增殖、凋亡和侵袭。总之，TGM2促进了LUSC的进展，靶向TGM2有望成为LUSC治疗的一种新的治疗方法。
