Sasani Ghamsari M, Arghavan M M
Photonics and Quantum Technologies Research School, Nuclear Science and Technology Research Institute, 11155-3436, Tehran, Iran.
Department of Physics, Payame Noor University, P.O.Box 19395-3697, Tehran, Iran.
Heliyon. 2024 Jun 15;10(12):e33139. doi: 10.1016/j.heliyon.2024.e33139. eCollection 2024 Jun 30.
The distinctive photophysical characteristics possessed by lanthanides, including europium, neodymium, and ytterbium, render them adaptable molecular tools for studying biological systems. Specifically, their enduring photoluminescence, precise emission spectra, and significant Stokes shifts allow for experiments not achievable with organic fluorophores or fluorescent proteins. Moreover, the capacity of these metal ions for luminescence resonance energy transfer and photon upconversion extends the potential applications of lanthanide probes even further. In this research, a new [Nd(NTA)2·HO] complex was synthesized and its optical properties were assessed using practical characterization techniques such as UV-Vis absorption, photoluminescence, and FTIR. It was discovered that when the sample was excited by a 357 nm wavelength, it emitted a strong line at 1076 nm with a full-width at half maximum (FWHM) of 10 nm, a phenomenon not previously documented. The Judd-Ofelt theory and its intensity parameters were utilized in a theoretical approach to determine the fluorescence branching ratio and the radiative lifetime of the [Nd(NTA)2·HO] complex. The absorption and luminescence spectra were then analyzed accordingly. Experimental findings validated the potential applications of the prepared sample in bioimaging.
包括铕、钕和镱在内的镧系元素所具有的独特光物理特性，使其成为研究生物系统的适用分子工具。具体而言，它们持久的光致发光、精确的发射光谱以及显著的斯托克斯位移，使得使用有机荧光团或荧光蛋白无法实现的实验成为可能。此外，这些金属离子的发光共振能量转移和光子上转换能力进一步扩展了镧系元素探针的潜在应用。在本研究中，合成了一种新的[Nd(NTA)2·HO]配合物，并使用紫外可见吸收、光致发光和傅里叶变换红外光谱等实际表征技术对其光学性质进行了评估。结果发现，当样品用357 nm波长激发时，它在1076 nm处发射出一条强谱线，半高宽（FWHM）为10 nm，这一现象此前未见报道。采用Judd-Ofelt理论及其强度参数，通过理论方法确定了[Nd(NTA)2·HO]配合物的荧光分支比和辐射寿命。然后据此对吸收光谱和发光光谱进行了分析。实验结果验证了所制备样品在生物成像中的潜在应用。
