Urban J H, Van de Kar L D, Schmitt S L, Brownfield M S
Life Sci. 1985 Oct 7;37(14):1335-42. doi: 10.1016/0024-3205(85)90249-8.
The present studies using kidney slices were designed to test whether serotonergic stimulation of renin secretion is mediated via an endocrine signal. Previous in vivo studies have indicated that central serotonergic neurons regulate renin secretion. Administration of the serotonin releaser dl-p-chloroamphetamine-HCl (PCA) to rats causes dose-dependent increases in renin secretion that can be blocked by serotonin depletion with p-chlorophenylalanine (PCPA), injections of 5,7-dihydroxytryptamine into the dorsal raphe nucleus or ablation of the mediobasal hypothalamus. The renin-releasing substance was obtained from nephrectomized male donor rats which were sacrificed 1 hour after receiving an injection of PCA intraperitoneally. Plasma from rats that received saline injections was used as control. The plasma was collected and separated by ultrafiltration into fractions containing solutes with molecular weights between 500-10,000 daltons. The renin-releasing ability of this substance was studied in vitro using rat renal cortical slices. The plasma fraction (M.W. = 500 - 10,000) from rats treated with PCA caused dose-dependent increases in renin release from the kidney slices. Heating of the plasma factor at 100 degrees C for 30 minutes did not reduce the ability of this substance to release renin from the kidney slices. PCA alone (66 X 10(-6)M) did not increase renin release from the kidney slices. These data suggest that stimulation of serotonergic receptors in the brain triggers the release of an endocrine factor that is capable of directly stimulating renin release from the kidneys.
本研究采用肾切片来测试肾素分泌的5-羟色胺能刺激是否通过内分泌信号介导。先前的体内研究表明，中枢5-羟色胺能神经元调节肾素分泌。给大鼠注射5-羟色胺释放剂dl-对氯苯丙胺盐酸盐（PCA）会导致肾素分泌呈剂量依赖性增加，这种增加可被对氯苯丙氨酸（PCPA）耗竭5-羟色胺、向中缝背核注射5,7-二羟基色胺或切除中基底下丘脑所阻断。肾素释放物质取自肾切除的雄性供体大鼠，这些大鼠在腹腔注射PCA 1小时后被处死。接受盐水注射的大鼠血浆用作对照。收集血浆并通过超滤分离成含有分子量在500 - 10,000道尔顿之间溶质的级分。使用大鼠肾皮质切片在体外研究该物质的肾素释放能力。PCA处理的大鼠的血浆级分（分子量 = 500 - 10,000）导致肾切片中肾素释放呈剂量依赖性增加。将血浆因子在100℃加热30分钟不会降低该物质从肾切片释放肾素的能力。单独使用PCA（66×10⁻⁶M）不会增加肾切片中肾素的释放。这些数据表明，刺激大脑中的5-羟色胺能受体会触发一种内分泌因子的释放，该因子能够直接刺激肾脏释放肾素。
