BACKGROUND

Lectins are carbohydrate-binding protein domains. The C-type lectin designates a requirement for calcium for binding. Proteins contain C-type lectin domains that have a diverse range of functions, including cell-cell adhesion, immune response to pathogens, and apoptosis. This study aimed to investigate the characters of LvCTL-encoding genes from white-leg shrimp ( ) in Central Vietnam.

METHODS

Two PCR products (LvCTL3 and LvCTL4) were cloned and sequenced. The structure and characterization of LvCTL proteins were predicted using bioinformatics tools.

RESULTS

The results showed that the gene was 444 nucleotides in length and 98.87% similar to the published gene (accession number: KF156943). The polypeptide sequence had 147 amino acids, which were 97.28% identical to the reference sequence (AGV68681) and the gene had a length of 417 nucleotides and homology of 99.52% compared to the published gene (KM387560). The deduced polypeptide sequence had 138 amino acids, and was 100% similar to the reference sequence (AKA64754). The had a molecular weight of 16.91 kDa and an isoelectric point (pI) of 4.66, while had 15.75 and 4.58 kDa, respectively. The structure prediction results showed that and had one domain (CTLD), had two α helices and nine β sheets, and had two α helices and eight β sheets.

CONCLUSIONS

Our results provide essential information for the heterologous expression and biosynthesis production of C-type lectins.