Hue University, Hue, 49000, Vietnam.
Faculty of Fisheries, Hue University of Agriculture and Forestry, Hue University, Hue City, Thua Thien Hue, 49000, Vietnam.
F1000Res. 2024 Jun 17;12:260. doi: 10.12688/f1000research.126044.3. eCollection 2023.
Lectins are carbohydrate-binding protein domains. The C-type lectin designates a requirement for calcium for binding. Proteins contain C-type lectin domains that have a diverse range of functions, including cell-cell adhesion, immune response to pathogens, and apoptosis. This study aimed to investigate the characters of LvCTL-encoding genes from white-leg shrimp ( ) in Central Vietnam.
Two PCR products (LvCTL3 and LvCTL4) were cloned and sequenced. The structure and characterization of LvCTL proteins were predicted using bioinformatics tools.
The results showed that the gene was 444 nucleotides in length and 98.87% similar to the published gene (accession number: KF156943). The polypeptide sequence had 147 amino acids, which were 97.28% identical to the reference sequence (AGV68681) and the gene had a length of 417 nucleotides and homology of 99.52% compared to the published gene (KM387560). The deduced polypeptide sequence had 138 amino acids, and was 100% similar to the reference sequence (AKA64754). The had a molecular weight of 16.91 kDa and an isoelectric point (pI) of 4.66, while had 15.75 and 4.58 kDa, respectively. The structure prediction results showed that and had one domain (CTLD), had two α helices and nine β sheets, and had two α helices and eight β sheets.
Our results provide essential information for the heterologous expression and biosynthesis production of C-type lectins.
凝集素是一类糖结合蛋白结构域。C 型凝集素指定需要钙结合。蛋白质包含 C 型凝集素结构域，具有多种功能，包括细胞-细胞黏附、对病原体的免疫反应和细胞凋亡。本研究旨在调查越南中部白对虾（）LvCTL 编码基因的特征。
克隆并测序了两个 PCR 产物（LvCTL3 和 LvCTL4）。使用生物信息学工具预测 LvCTL 蛋白的结构和特征。
结果表明，基因长 444 个核苷酸，与已发表的基因（登录号：KF156943）相似，相似度为 98.87%。多肽序列有 147 个氨基酸，与参考序列（AGV68681）的相似度为 97.28%，与已发表的基因（KM387560）的相似度为 99.52%。推导的多肽序列有 138 个氨基酸，与参考序列（AKA64754）的相似度为 100%。基因的分子量为 16.91 kDa，等电点（pI）为 4.66，而基因的分子量分别为 15.75 kDa 和 4.58 kDa。结构预测结果表明，和具有一个结构域（CTLD），具有两个α螺旋和九个β片层，而具有两个α螺旋和八个β片层。
本研究结果为 C 型凝集素的异源表达和生物合成生产提供了重要信息。
