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有证据表明霍乱弧菌中存在一种类似于大肠杆菌recA系统的系统。

Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae.

作者信息

Ghosh R K, Siddiqui K A, Mukhopadhyay G, Ghosh A

出版信息

Mol Gen Genet. 1985;200(3):439-41. doi: 10.1007/BF00425728.

Abstract

Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coli. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lytic growth of a clear plaque mutant of the phage was affected by Antipain.

摘要

有两条证据表明,霍乱弧菌中存在一种与大肠杆菌recA基因类似的基因,并且其产物在SOS反应中发挥蛋白水解功能。首先,以大肠杆菌的recA基因为探针进行Southern印迹杂交,结果显示霍乱弧菌中有一个基因组序列与该探针杂交。其次,像呋喃唑酮这样的DNA损伤剂引发的霍乱弧菌中的类SOS反应(通过β噬菌体诱导来测定),可以被抗蛋白酶抑制,抗蛋白酶是一种已知能抑制大肠杆菌中RecA蛋白酶作用的蛋白酶抑制剂。抗蛋白酶对β噬菌体诱导的最大阻断作用发生在1 mM时。在此浓度下,抗蛋白酶既不影响宿主细菌的活力,也不影响噬菌体清晰噬菌斑突变体的裂解生长。

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