Meng Xiang-Rui, Gan Yu, Liao Li-Jun, Li Chao-Nan, Wang Rong, Liu Mei, Deng Jun-Yin, Chen Yan
Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang, China.
Zhejiang Tiantong Forest Ecosystem National Observation and Research Station, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, China.
Front Plant Sci. 2024 Jun 28;15:1374431. doi: 10.3389/fpls.2024.1374431. eCollection 2024.
Plant-parasitic nematodes (PPNs) are among the most damaging pathogens to host plants. Plants can modulate their associated bacteria to cope with nematode infections. The tritrophic plant-nematode-microbe interactions are highly taxa-dependent, resulting in the effectiveness of nematode agents being variable among different host plants. is a versatile plant with high application potential for fruits or medicines. In recent years, a few farmers have attempted to cultivate this species in Sichuan, China, where parasitic nematodes are present. We used 16S rRNA genes to explore the effects of nematode parasitism on root-associated bacteria in this species. Our results revealed that nematode infection had effects on both endophytic bacterial communities and rhizosphere communities in roots, but on different levels. The species richness increased in the rhizosphere bacterial communities of infected individuals, but the community composition remained similar as compared with that of healthy individuals. Nematode infection induces a deterministic assembly process in the endophytic bacterial communities of parasitized organs. Significant taxonomic and functional changes were observed in the endophytic communities of root knots. These changes were characterized by the enrichment of nitrogen-fixing bacteria, including , , and nematode-antagonistic bacteria, such as , , , , and . Our results would help the understanding of the tritrophic plant-nematode-bacterium interactions in host plants other than dominant crops and vegetables and would provide essential information for successful nematode management when were cultivated on large scales.
植物寄生线虫（PPNs）是对寄主植物危害最大的病原体之一。植物可以调节其相关细菌以应对线虫感染。植物-线虫-微生物的三营养相互作用高度依赖于分类群，导致线虫制剂在不同寄主植物中的有效性存在差异。[植物名称]是一种用途广泛的植物，在水果或医药方面具有很高的应用潜力。近年来，一些农民试图在中国四川种植这种植物，而该地区存在寄生线虫。我们使用16S rRNA基因来探究线虫寄生对该植物根系相关细菌的影响。我们的结果表明，线虫感染对[植物名称]根系的内生细菌群落和根际群落均有影响，但程度不同。受感染个体的根际细菌群落物种丰富度增加，但与健康个体相比，群落组成保持相似。线虫感染在被寄生器官的内生细菌群落中诱导了一个确定性的组装过程。在根结的内生群落中观察到了显著的分类学和功能变化。这些变化的特征是固氮细菌的富集，包括[具体固氮细菌名称]，以及对线虫有拮抗作用的细菌，如[具体拮抗细菌名称]。我们的结果将有助于理解除主要作物和蔬菜以外的寄主植物中植物-线虫-细菌的三营养相互作用，并为大规模种植[植物名称]时成功进行线虫管理提供重要信息。
