Department of Ophthalmology, Edward S. Harkness Eye Institute, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA.
Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Transl Vis Sci Technol. 2024 Jul 1;13(7):12. doi: 10.1167/tvst.13.7.12.
The purpose of this study was to evaluate the impact of vitrectomy and posterior hyaloid (PH) peeling on color alteration of optic nerve head (ONH) and retina as a surrogate biomarker of induced perfusion changes.
Masked morphometric and colorimetric analyses were conducted on preoperative (<1 month) and postoperative (<18 months) color fundus photographs of 54 patients undergoing vitrectomy, either with (44) or without (10) PH peeling and 31 years of age and gender-matched control eyes. Images were calibrated according to the hue and saturation values of the parapapillary venous blood column. Chromatic spectra of the retinal pigment epithelium and choroid were subtracted to avoid color aberrations. Red, green, and blue (RGB) bit values over the ONH and retina were plotted within the constructed RGB color space to analyze vitrectomy-induced color shift. Vitrectomy-induced parapapillary vein caliber changes were also computed morphometrically.
A significant post-vitrectomy red hue shift was noted on the ONH (37.1 degrees ± 10.9 degrees vs. 4.1 degrees ± 17.7 degrees, P < 0.001), which indicates a 2.8-fold increase in blood perfusion compared to control (2.6 ± 1.9 vs. 0.9 ± 1.8, P < 0.001). A significant post-vitrectomy increase in the retinal vein diameter was also noticed (6.8 ± 6.4% vs. 0.1 ± 0.3%, P < 0.001), which was more pronounced with PH peeling (7.9 ± 6.6% vs. 3.1 ± 4.2%, P = 0.002).
Vitrectomy and PH peeling increase ONH and retinal blood flow. Colorimetric and morphometric analyses offer valuable insights for future artificial intelligence and deep learning applications in this field.
The methodology described herein can easily be applied in different clinical settings and may enlighten the beneficial effects of vitrectomy in several retinal vascular diseases.
本研究旨在评估玻璃体切除术和后玻璃体（PH）剥离对视神经头（ONH）和视网膜颜色改变的影响，将其作为诱导灌注变化的替代生物标志物。
对 54 例接受玻璃体切除术的患者（44 例伴 PH 剥离，10 例不伴 PH 剥离）和 31 岁年龄和性别匹配的对照组眼术前（<1 个月）和术后（<18 个月）的眼底彩照进行了掩蔽形态计量学和比色分析。根据视盘旁静脉血柱的色调和饱和度值对图像进行校准。减去视网膜色素上皮和脉络膜的色光谱以避免色像差。在构建的 RGB 彩色空间内绘制 ONH 和视网膜上的红、绿、蓝（RGB）位值，以分析玻璃体切除术后引起的颜色移位。还通过形态计量学计算了玻璃体切除术后视盘旁静脉口径的变化。
在 ONH 上观察到玻璃体切除术后明显的红色色调偏移（37.1°±10.9°vs. 4.1°±17.7°，P<0.001），这表明与对照组相比（2.6±1.9 比 0.9±1.8，P<0.001），血流灌注增加了 2.8 倍。还观察到玻璃体切除术后视网膜静脉直径显著增加（6.8%±6.4%比 0.1%±0.3%，P<0.001），在 PH 剥离时更为明显（7.9%±6.6%比 3.1%±4.2%，P=0.002）。
玻璃体切除术和 PH 剥离增加了 ONH 和视网膜血流。比色和形态计量分析为该领域未来的人工智能和深度学习应用提供了有价值的见解。
张宇轩
