Nikkinen P, Räisänen J, Hänninen R, Hyvönen-Dabek M, Dabek J T
Phys Med Biol. 1985 Sep;30(9):921-8. doi: 10.1088/0031-9155/30/9/004.
The total nitrogen concentrations in dried serum from 54 pregnant women and 17 newborn babies were determined by a new application of the 14N(p,p' gamma)14N reaction resonance at 3.9 MeV. The samples were bombarded in a He atmosphere by 4.1 MeV protons from a tandem Van de Graaff accelerator. The mean dry-weight nitrogen concentration in serum sampled during early pregnancy (6-12 weeks; 13.9 g per 100 g +/- 5.4%) was significantly higher than that in serum sampled during late pregnancy (38-42 weeks: 13.0 g per 100 g +/- 3.9%, p less than 0.001) and than that in serum taken from the umbilical cord (13.3 g per 100 g +/- 4.6%, p less than 0.01). The nitrogen levels measured using this rapid nuclear technique, applied for the first time to human serum analysis, agree well with parallel Kjeldahl analyses.
采用3.9兆电子伏特的14N(p,p'γ)14N反应共振的新应用方法，测定了54名孕妇和17名新生儿干燥血清中的总氮浓度。样品在氦气氛围中，用来自串联范德格拉夫加速器的4.1兆电子伏特质子进行轰击。孕早期（6 - 12周；每100克13.9克±5.4%）采集的血清中，平均干重氮浓度显著高于孕晚期（38 - 42周：每100克13.0克±3.9%，p<0.001）采集的血清，也高于脐带血血清（每100克13.3克±4.6%，p<0.01）。首次应用于人体血清分析的这种快速核技术所测得的氮水平，与凯氏定氮法平行分析结果吻合良好。
