Moazezi Ghavihelm Ali, Nabian Sedigheh, Jamshidi Shahram, Taheri Mohammad, Soltani Minoo, Mazaheri Nezhad Fard Ramin, Akbari Pazoki Ali
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Parasitol. 2024 Apr-Jun;19(2):153-161. doi: 10.18502/ijpa.v19i2.15851.
is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin () and proteins against and for monocyte-derived exosome preparation.
This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of and using various immunoinformatics and bioinformatics approaches. β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.
This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.
A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further and studies.
利什曼原虫是一种媒介传播的原生动物，可导致人和动物的内脏、皮肤及黏膜皮肤利什曼病。单核细胞衍生的外泌体疫苗可作为预防和免疫治疗策略。本研究的目的是利用利什曼溶素（）和蛋白设计一种多表位候选疫苗，用于制备单核细胞衍生的外泌体以对抗利什曼原虫。
本研究于2023 - 2024年在伊朗德黑兰大学兽医学院开展。使用多种免疫信息学和生物信息学方法从利什曼原虫的两种抗原蛋白中筛选出有效的免疫显性表位。β亚基用作佐剂以刺激免疫反应。然后，选择合适的连接子用于表位融合。对候选疫苗的三维模型进行了预测和验证。
这种设计的候选疫苗可有效用作预防利什曼病的策略。
通过生物信息学和免疫信息学研究设计了一种质量在虚拟层面可接受的候选疫苗；然而，该疫苗的有效性应通过进一步的体内和体外研究加以验证。
