Nauck M, van Hoorn W, Gubernatis G, Ebert R, Siewert J R, Creutzfeldt W
Res Exp Med (Berl). 1985;185(4):291-8. doi: 10.1007/BF01851954.
Plasma insulin responses to intragastric (i.g.) (1.5 g/kg b.wt.) and "isoglycemic" intravenous (i.v.) glucose were measured in ten unanesthetized young pigs to assess the contribution of gastrointestinal factors to the total insulin secretion as observed after i.g. glucose. The participation of nerves was estimated by comparing metabolic tests performed before and after total surgical pancreatic denervation. In the five animals which survived the procedure, 52.6% of the insulin response after i.g. glucose was calculated to be due to incretion factors, a value similar to the 54.8% found in the preoperative series (with intact pancreatic innervation). The response of IR-GIP to i.g. glucose was not significantly different between preoperative and postoperative tests, although a subtotal duodenectomy had to be performed in the course of the operation designed to completely denervate the pancreas. Intragastric and i.v. (also tested by bolus glucose injection) glucose tolerance was almost identical before and after the operation. It was concluded that nerves do not seem to play a major role in mediating the incretin effect in pigs. Hormonal factors, including GIP, appear to be more important.
在十头未麻醉的幼猪中测量了血浆胰岛素对胃内（i.g.）（1.5 g/kg体重）和“等血糖”静脉内（i.v.）葡萄糖的反应，以评估胃肠道因素对胃内葡萄糖后观察到的总胰岛素分泌的贡献。通过比较全胰手术去神经前后进行的代谢测试来估计神经的参与情况。在手术存活的五只动物中，计算出胃内葡萄糖后胰岛素反应的52.6%归因于肠促胰岛素因子，这一数值与术前系列（胰腺神经完整）中发现的54.8%相似。尽管在旨在完全去神经胰腺的手术过程中必须进行次全十二指肠切除术，但术前和术后测试中IR-GIP对胃内葡萄糖的反应没有显著差异。手术前后胃内和静脉内（也通过推注葡萄糖注射进行测试）的葡萄糖耐量几乎相同。得出的结论是，神经似乎在介导猪的肠促胰岛素效应中不起主要作用。包括GIP在内的激素因素似乎更为重要。
