Ghinea E, Dumitriu L, Handoca A, Damian A, Stefanovici G, Ciocîrdia C, Oprescu M, Pop A
Endocrinologie. 1985 Jul-Sep;23(3):155-67.
The authors have studied the reactivity of cold thyroid nodule cells (CN) by comparison with the reactivity of normal thyroid cells (Ty), hot nodules cells (N), Graves' disease cells (G) and cells from the area proximal to cold nodules (TyC), under the influence of increased amounts of T3, TSH, STH, insulin, estradiol and KI present in the culture medium. The experiment lasted for 9-10 days. The study was carried out on monolayer cultures of cells obtained by tripsinization and on organocultures. Proteic synthesis, cytochemical and cytoenzymatic activities in the culture under the influence of the above substances were examined comparatively. After interruption of the treatment, the monolayer cultures were stained with Giemsa and examined by light microscopy. Proteic synthesis and release of Tg, T3 and T4 into the culture medium, were also examined. The organocultures were used in evaluating 125I uptake from the medium under the influence of the treatment. The results showed variation in relation to the tissue and hormone dose applied. It was especially found that TSH and estradiol cause enlargement of CN cell nuclei and an increased number of mitoses and polyploidia which might be premises for possible malignization.
作者通过将冷甲状腺结节细胞（CN）与正常甲状腺细胞（Ty）、热结节细胞（N）、格雷夫斯病细胞（G）以及冷结节近端区域的细胞（TyC）的反应性进行比较，研究了在培养基中添加过量的T3、促甲状腺激素（TSH）、生长激素（STH）、胰岛素、雌二醇和碘化钾（KI）的影响下，冷甲状腺结节细胞的反应性。实验持续了9 - 10天。该研究在通过胰蛋白酶消化获得的细胞单层培养物以及器官培养物上进行。对上述物质影响下培养物中的蛋白质合成、细胞化学和细胞酶活性进行了比较检查。处理中断后，将单层培养物用吉姆萨染色并通过光学显微镜检查。还检查了蛋白质合成以及甲状腺球蛋白（Tg）、T3和T4释放到培养基中的情况。器官培养物用于评估处理影响下培养基中碘 - 125（125I）的摄取情况。结果显示，这与所应用的组织和激素剂量有关。特别发现促甲状腺激素和雌二醇会导致冷甲状腺结节细胞核增大、有丝分裂和多倍体数量增加，这可能是潜在恶性变的前提。
