Ghinea E, Simionescu L, Dimitriu V
Endocrinologie. 1983 Jan-Mar;21(1):29-35.
The experiments were carried out on 26 lots of thyroid cell cultures treated with sodium sulphate, lithium sulphate, lithium carbonate, cystine, cysteine, and methionine, in 2 doses each, in the presence or absence of iodine in the culture medium. The monolayer culture was used in morphologic, cytochemical and cytoenzymatic studies, and the culture medium was used for assay of thyroglobulin and thyroxin. We found that addition of aminoacids with SH or S--S groups to the culture medium causes stimulation of protein synthesis overstrain on the oxydases system at the expense of dehydrogenases. Stimulation of protein synthesis in the thyroid cell cultures under study is not accompanied by a corresponding hormonal secretory activity, the process of hormonal biosynthesis being inhibited as compared to the control culture.
实验是在26批用硫酸钠、硫酸锂、碳酸锂、胱氨酸、半胱氨酸和蛋氨酸处理的甲状腺细胞培养物上进行的，每种物质各有2种剂量，培养基中存在或不存在碘。单层培养用于形态学、细胞化学和细胞酶学研究，培养基用于甲状腺球蛋白和甲状腺素的测定。我们发现，向培养基中添加具有SH或S-S基团的氨基酸会刺激蛋白质合成，以脱氢酶为代价使氧化酶系统过度紧张。在所研究的甲状腺细胞培养物中，蛋白质合成的刺激并未伴随着相应的激素分泌活性，与对照培养物相比，激素生物合成过程受到抑制。
