Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil.
Laboratório de Baculovírus, Instituto de Ciências Biológicas, Universidade de Brasília (UnB), Brasília, Brasil.
Braz J Microbiol. 2024 Sep;55(3):2893-2900. doi: 10.1007/s42770-024-01449-0. Epub 2024 Jul 16.
Water buffalo (Bubalus bubalis) farming is increasing in many regions of the world due to the species' ability to thrive in environments where bovine cattle would struggle. Despite water buffaloes being known for their resistance to diseases, there is a lack of data about the diversity of the microbiome of the species. In this study, we examined the virome diversity in palatine tonsils collected from animals from the island of Marajó, northern Pará state, Brazil, which harbors the largest bubaline flock in the country. Tonsil fragments from 60 clinically healthy bubalines were randomly selected from a sample of 293 animals. The samples were purified, extracted, and randomly amplified with phi29 DNA polymerase. After amplification, the products were purified and sequenced. Circular DNA viruses were predominant in the tonsils' virome. Sequences of genome segments representative of members of the genera Alphapolyomavirus (including a previously unreported bubaline polyomavirus genome) and Gemycircularvirus were identified, along with other not yet classified circular virus genomes. As the animals were clinically healthy at the time of sampling, such viruses likely constitute part of the normal tonsillar virome of water buffaloes inhabiting the Ilha do Marajó biome.
水 buffalo（Bubalus bubalis）养殖在世界许多地区都在增加，因为该物种能够在牛难以生存的环境中茁壮成长。尽管水 buffalo 以其对疾病的抵抗力而闻名，但有关该物种微生物组多样性的数据却很少。在这项研究中，我们检查了来自巴西帕拉州北部马拉若岛的动物的扁桃体中病毒组的多样性，该岛拥有该国最大的 bubaline 群。从 293 只动物的样本中随机选择了 60 只临床健康的 bubaline 的扁桃体碎片。对样本进行了纯化、提取和随机扩增，使用 phi29 DNA 聚合酶进行扩增。扩增后，对产物进行了纯化和测序。扁桃体病毒组中主要存在环状 DNA 病毒。鉴定出代表 Alphapolyomavirus 属成员（包括以前未报道的 bubaline 多瘤病毒基因组）和 Gemycircularvirus 的基因组片段序列，以及其他尚未分类的环状病毒基因组。由于在采样时动物是临床健康的，因此这些病毒可能构成居住在马拉若岛生物群落的水 buffalo 正常扁桃体病毒组的一部分。
