Zhong Jian, Zhang Jinyi, Zhang Yadong, Ge Yang, He Wenjing, Liang Chengjuan, Gao Yulin, Zhu Zengrong, Machado Ricardo A R, Zhou Wenwu
State Key Laboratory of Rice Biology and Breeding, Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
Hainan Institute, Zhejiang University, Sanya, 572000, China.
BMC Plant Biol. 2024 Jul 17;24(1):677. doi: 10.1186/s12870-024-05404-x.
Climate change is predicted to increase the occurrence of extreme weather events such as heatwaves, which may thereby impact the outcome of plant-herbivore interactions. While elevated temperature is known to directly affect herbivore growth, it remains largely unclear if it indirectly influences herbivore performance by affecting the host plant they feed on. In this study, we investigated how transient exposure to high temperature influences plant herbivory-induced defenses at the transcript and metabolic level. To this end, we studied the interaction between potato (Solanum tuberosum) plants and the larvae of the potato tuber moth (Phthorimaea operculella) under different temperature regimes. We found that P. operculella larvae grew heavier on leaves co-stressed by high temperature and insect herbivory than on leaves pre-stressed by herbivory alone. We also observed that high temperature treatments altered phylotranscriptomic patterns upon herbivory, which changed from an evolutionary hourglass pattern, in which transcriptomic responses at early and late time points after elicitation are more variable than the ones in the middle, to a vase pattern. Specifically, transcripts of many herbivory-induced genes in the early and late defense stage were suppressed by HT treatment, whereas those in the intermediate stage peaked earlier. Additionally, we observed that high temperature impaired the induction of jasmonates and defense compounds upon herbivory. Moreover, using jasmonate-reduced (JA-reduced, irAOC) and -elevated (JA-Ile-elevated, irCYP94B3s) potato plants, we showed that high temperature suppresses JA signaling mediated plant-induced defense to herbivore attack. Thus, our study provides evidences on how temperature reprograms plant-induced defense to herbivores.
据预测，气候变化将增加极端天气事件的发生频率，如热浪，这可能会影响植物与食草动物之间相互作用的结果。虽然已知温度升高会直接影响食草动物的生长，但温度是否会通过影响其取食的寄主植物而间接影响食草动物的表现，目前仍不清楚。在本研究中，我们调查了短暂暴露于高温如何在转录和代谢水平上影响植物对食草动物取食的诱导防御。为此，我们研究了马铃薯（Solanum tuberosum）植株与马铃薯块茎蛾（Phthorimaea operculella）幼虫在不同温度条件下的相互作用。我们发现，与仅受食草动物取食预处理的叶片相比，马铃薯块茎蛾幼虫在同时受到高温和昆虫取食胁迫的叶片上生长得更重。我们还观察到，高温处理改变了取食后的系统转录组模式，从进化沙漏模式（诱导后早期和晚期的转录组反应比中期更具变异性）转变为花瓶模式。具体而言，高温处理抑制了早期和晚期防御阶段许多食草动物诱导基因的转录本，而中期的转录本峰值出现得更早。此外，我们观察到高温削弱了取食后茉莉酸和防御化合物的诱导。此外，使用茉莉酸减少（JA减少，irAOC）和升高（茉莉酸异亮氨酸升高，irCYP94B3s）的马铃薯植株，我们表明高温抑制了茉莉酸信号介导的植物对食草动物攻击的诱导防御。因此，我们的研究为温度如何重新编程植物对食草动物的诱导防御提供了证据。
