Al-Asfour Adel, Karched Maribasappa, Qasim Syed Saad Bin, Zafiropoulos Gregor-Georg
Department of Surgical Sciences, College of Dentistry, Kuwait University, Safat, Kuwait.
Department of Bioclinical Sciences, College of Dentistry, Kuwait University, Safat, Kuwait.
Clin Exp Dent Res. 2024 Aug;10(4):e902. doi: 10.1002/cre2.902.
Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of Candida albicans on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.
Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. C. albicans (ATCC 24433) was cultured for 24 h, and its cell surface hydrophobicity was assessed using a modified method. C. albicans adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).
C. albicans was found to be hydrophobic (77.25%). SEM analysis showed that C. albicans adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real-time PCR quantification revealed significantly greater adhesion of C. albicans cells to PTFE membranes than the control membrane (p ≤ .001) with the membranes having a textured surface exhibiting the highest count of 2680 × 10 cells/ml compared to the count of 707 × 10 cells/mL on those with a nontextured one (p ≤ .001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010 × 10 cells/ml (p ≤ .05).
The results of this study indicate that C. albicans adhesion on membranes' surfaces depends on the degree of surface roughness and/or on the presence of a texture. Textured PTFE membranes and/or membranes high roughness showed significantly more adhered C. albicans cells. These findings can impact the surgeon's choice of GBR membrane and postoperative maintenance.
引导骨再生（GBR）是用于修复骨缺损的核心手术。本研究旨在调查白色念珠菌在GBR手术中使用的六种市售聚四氟乙烯（PTFE）膜上的黏附情况以及随后的临床后果。
测试了六种市售PTFE膜。其中两种膜具有纹理表面，另外四种具有平面、无纹理表面。白色念珠菌（ATCC 24433）培养24小时，使用改良方法评估其细胞表面疏水性。通过扫描电子显微镜（SEM）和实时聚合酶链反应（PCR）研究白色念珠菌对膜片的黏附。
发现白色念珠菌具有疏水性（77.25%）。SEM分析表明，除了一种表面最粗糙的无纹理膜上观察到厚生物膜外，白色念珠菌对所有检查的膜的黏附特征为片状、散在和小簇状模式。实时PCR定量显示，白色念珠菌细胞对PTFE膜的黏附明显高于对照膜（p≤0.001），具有纹理表面的膜的黏附细胞计数最高，为2680×10个细胞/毫升，而无纹理表面的膜的计数为707×10个细胞/毫升（p≤0.001）。发现一种无纹理表面但最粗糙的膜的黏附细胞计数最高，为3010×10个细胞/毫升（p≤0.05）。
本研究结果表明，白色念珠菌在膜表面的黏附取决于表面粗糙度程度和/或纹理的存在。有纹理的PTFE膜和/或高粗糙度的膜显示出明显更多的白色念珠菌黏附细胞。这些发现可能会影响外科医生对GBR膜的选择和术后维护。
