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人类手指神经近端和远端损伤部位的定量质谱分析。

Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends.

作者信息

Frostadottir Drifa, Welinder Charlotte, Perez Raquel, Dahlin Lars B

机构信息

Department of Translational Medicine - Hand Surgery, Lund University, Malmö, Sweden.

Department of Hand Surgery, Skåne University Hospital, Malmö, Sweden.

出版信息

Front Mol Neurosci. 2024 Jul 2;17:1425780. doi: 10.3389/fnmol.2024.1425780. eCollection 2024.

DOI:10.3389/fnmol.2024.1425780
PMID:39015129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11250671/
Abstract

INTRODUCTION

Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, focusing on differences in protein expression between the proximal and distal nerve ends.

METHODS

A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30-66] years), harvested during primary nerve repair within 48 h post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry.

RESULTS

A total of 3,914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal and the distal nerve ends. The downregulation of proteins in the distal nerve end was associated with synaptic transmission, autophagy, neurotransmitter regulation, cell adhesion and migration. Conversely, proteins upregulated in the distal nerve end were implicated in cellular stress response, neuromuscular junction stability and muscle contraction, neuronal excitability and neurotransmitter release, synaptic vesicle recycling and axon guidance and angiogenesis.

DISCUSSION

Investigation of proteins, with functional annotations analysis, in proximal and the distal ends of human injured digital nerves, revealed dynamic cellular responses aimed at promoting tissue degeneration and restoration, while suppressing non-essential processes.

摘要

引言

对受伤的人类周围神经进行蛋白质组学分析，尤其是关注近端和远端神经末梢发生的事件，目前仍相对缺乏研究。本研究旨在调查指神经损伤背后的分子模式，重点关注近端和远端神经末梢之间蛋白质表达的差异。

方法

共收集了26例人类指神经损伤样本（24名男性；2名女性；中位年龄47[30-66]岁），在损伤后48小时内进行一期神经修复时从近端和远端神经末梢采集，使用质谱分析。

结果

共鉴定出3914种蛋白质，其中127种蛋白质在近端和远端神经末梢的丰度上存在显著差异。远端神经末梢中蛋白质的下调与突触传递、自噬、神经递质调节、细胞粘附和迁移有关。相反，远端神经末梢中上调的蛋白质与细胞应激反应、神经肌肉接头稳定性和肌肉收缩、神经元兴奋性和神经递质释放、突触小泡循环以及轴突导向和血管生成有关。

讨论

对人类受伤指神经近端和远端的蛋白质进行功能注释分析，揭示了旨在促进组织退化和修复同时抑制非必要过程的动态细胞反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/fa5ad0209efd/fnmol-17-1425780-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/565811a6b806/fnmol-17-1425780-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/fa5ad0209efd/fnmol-17-1425780-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/565811a6b806/fnmol-17-1425780-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/ee9eed1a8f4d/fnmol-17-1425780-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d201/11250671/16a35a68757b/fnmol-17-1425780-g003.jpg
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本文引用的文献

1
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Handb Clin Neurol. 2024;201:1-17. doi: 10.1016/B978-0-323-90108-6.00014-4.
2
Genes in Axonal Regeneration.轴突再生中的基因。
Mol Neurobiol. 2024 Oct;61(10):7431-7447. doi: 10.1007/s12035-024-04049-z. Epub 2024 Feb 22.
3
The Dynamics of Nerve Degeneration and Regeneration in a Healthy Milieu and in Diabetes.在健康环境和糖尿病中神经变性和再生的动态。
Int J Mol Sci. 2023 Oct 16;24(20):15241. doi: 10.3390/ijms242015241.
4
Calpain signaling: from biology to therapeutic opportunities in neurodegenerative disorders.钙蛋白酶信号传导：从生物学原理到神经退行性疾病的治疗契机
Front Vet Sci. 2023 Sep 5;10:1235163. doi: 10.3389/fvets.2023.1235163. eCollection 2023.
5
Quantification of heat shock proteins in the posterior interosseous nerve among subjects with type 1 and type 2 diabetes compared to healthy controls.与健康对照相比，1型和2型糖尿病患者骨间后神经中热休克蛋白的定量分析。
Front Neurosci. 2023 Aug 8;17:1227557. doi: 10.3389/fnins.2023.1227557. eCollection 2023.
6
Human FAM3C restores memory-based thermotaxis of loss-of-function mutants.人类FAM3C可恢复功能缺失突变体基于记忆的趋温性。
PNAS Nexus. 2022 Oct 25;1(5):pgac242. doi: 10.1093/pnasnexus/pgac242. eCollection 2022 Nov.
7
The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regeneration.损伤坐骨神经图谱(iSNAT)：深入了解神经组织退化和再生的细胞和分子基础。
Elife. 2022 Dec 14;11:e80881. doi: 10.7554/eLife.80881.
8
Common Patterns of Hydrolysis Initiation in P-loop Fold Nucleoside Triphosphatases.P 环折叠核苷三磷酸酶中水解起始的常见模式。
Biomolecules. 2022 Sep 22;12(10):1345. doi: 10.3390/biom12101345.
9
Loss of Stathmin-2, a hallmark of TDP-43-associated ALS, causes motor neuropathy.TDP-43 相关肌萎缩侧索硬化症的标志性蛋白 Stathmin-2 的缺失可导致运动性神经病。
Cell Rep. 2022 Jun 28;39(13):111001. doi: 10.1016/j.celrep.2022.111001.
10
DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update).DAVID：一个用于基因列表功能富集分析和功能注释的网络服务器（2021 更新）。
Nucleic Acids Res. 2022 Jul 5;50(W1):W216-W221. doi: 10.1093/nar/gkac194.