Jesus Rainde Naiara Rezende de, Tsatsanis Christos, Moura Camilla Christian Gomes, Zanetta-Barbosa Darceny, Stavropoulos Andreas
Malmö University - MAU, Faculty of Odontology, Department of Periodontology, Malmö, Sweden.
University of Crete - UOC, School of Medicine, Department of Clinical Chemistry, Heraklion, Crete, Greece.
Braz Oral Res. 2024 Jul 12;38:e064. doi: 10.1590/1807-3107bor-2024.vol38.0064. eCollection 2024.
The aim of this study was to evaluate the influence of implant macrodesign and surface hydrophilicity on osteoclast (OC) differentiation, activation, and survival in vitro. Titanium disks were produced with a sandblasted, dual acid-etched surface, with or without additional chemical modification for increasing hydrophilicity (SAE-HD and SAE, respectively) and different macrodesign comprising trapezoidal (HLX) or triangular threads (TMX). This study evaluated 7 groups in total, 4 of which were experimental: HLX/SAE-HD, HLX-SAE, TMX/SAE-HD, and TMX/SAE; and 3 control groups comprising OC differentiated on polystyrene plates (CCPC): a positive CCPC (+), a negative CCPC (-), and a lipopolysaccharide-stimulated assay positive control group, CCPC-LPS. Murine macrophage RAW264.7 cells were seeded on the disks, differentiated to OC (RAW-OC) by receptor activator of nuclear factor-κB ligand (RANKL) treatment and cultured for 5 days. Osteoclast differentiation and cell viability were respectively assessed by specific enzymatic Tartrate-Resistant Acid Phosphatase (TRAP) activity and MTT assays. Expression levels of various OC-related genes were measured at the mRNA level by quantitative polymerase chain reaction (qPCR). HLX/SAE-HD, TMX/SAE-HD, and HLX/SAE significantly suppressed OC differentiation when compared to CCPC (+). Cell viability was significantly increased in TMX/SAE and reduced in HLX/SAE-HD. In addition, the expression of Interleukin (IL)-6 and Tumour Necrosis Factor (TNF)-α was upregulated in TMX/SAE-HD compared to CCPC (+). Hydrophilic surfaces negatively modulate macrophage/osteoclast viability. Specifically, SAE-HD with double triangular threads increases the cellular pro-inflammatory status, while surface hydrophilicity and macrodesign do not seem to have a distinct impact on osteoclast differentiation, activation, or survival.
本研究的目的是评估种植体宏观设计和表面亲水性对体外破骨细胞（OC）分化、激活和存活的影响。制备了具有喷砂、双重酸蚀表面的钛盘，分别进行或不进行增加亲水性的额外化学修饰（分别为SAE-HD和SAE），以及具有不同宏观设计的梯形（HLX）或三角形螺纹（TMX）。本研究共评估了7组，其中4组为实验组：HLX/SAE-HD、HLX-SAE、TMX/SAE-HD和TMX/SAE；3个对照组包括在聚苯乙烯板上分化的OC（CCPC）：阳性CCPC（+）、阴性CCPC（-）和脂多糖刺激试验阳性对照组CCPC-LPS。将小鼠巨噬细胞RAW264.7细胞接种在钛盘上，通过核因子κB受体激活剂配体（RANKL）处理使其分化为OC（RAW-OC），并培养5天。分别通过特异性酶抗酒石酸酸性磷酸酶（TRAP）活性和MTT试验评估破骨细胞分化和细胞活力。通过定量聚合酶链反应（qPCR）在mRNA水平测量各种OC相关基因的表达水平。与CCPC（+）相比，HLX/SAE-HD、TMX/SAE-HD和HLX/SAE显著抑制OC分化。TMX/SAE组细胞活力显著增加，HLX/SAE-HD组细胞活力降低。此外，与CCPC（+）相比，TMX/SAE-HD组白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α的表达上调。亲水性表面对巨噬细胞/破骨细胞活力具有负调节作用。具体而言，具有双三角形螺纹的SAE-HD会增加细胞促炎状态，而表面亲水性和宏观设计似乎对破骨细胞分化、激活或存活没有明显影响。
