Institution of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, Zhejiang 315000, PR China.
Mol Pharm. 2024 Aug 5;21(8):3936-3950. doi: 10.1021/acs.molpharmaceut.4c00295. Epub 2024 Jul 17.
Micro- and nanoparticles delivery systems have been widely studied as vaccine adjuvants to enhance immunogenicity and sustain long-term immune responses. Polygonatum sibiricum polysaccharide (PSP) has been widely studied as an immunoregulator in improving immune responses. In this study, we synthesized and characterized cationic modified calcium carbonate (CaCO) microparticles loaded with PSP (PEI-PSP-CaCO, CTAB-PSP-CaCO), studied the immune responses elicited by PEI-PSP-CaCO and CTAB-PSP-CaCO carrying ovalbumin (OVA). Our results demonstrated that PEI-PSP-CaCO significantly enhanced the secretion of IgG and cytokines (IL-4, IL-6, IFN-γ, and TNF-α) in vaccinated mice. Additionally, PEI-PSP-CaCO induced the activation of dendritic cells (DCs), T cells, and germinal center (GC) B cells in draining lymph nodes (dLNs). It also enhanced lymphocyte proliferation, increased the ratio of CD4/CD8 T cells, and elevated the frequency of CD3 CD69 T cells in spleen lymphocytes. Therefore, PEI-PSP-CaCO microparticles induced a stronger cellular and humoral immune response and could be potentially useful as a vaccine delivery and adjuvant system.
作为疫苗佐剂，微纳米颗粒递药系统已被广泛研究以增强免疫原性和维持长期免疫应答。玉竹多糖（PSP）已被广泛研究作为一种免疫调节剂，以改善免疫应答。在本研究中，我们合成并表征了负载 PSP 的阳离子改性碳酸钙（CaCO）微粒（PEI-PSP-CaCO、CTAB-PSP-CaCO），研究了载有卵清蛋白（OVA）的 PEI-PSP-CaCO 和 CTAB-PSP-CaCO 引发的免疫应答。结果表明，PEI-PSP-CaCO 显著增强了接种小鼠 IgG 和细胞因子（IL-4、IL-6、IFN-γ 和 TNF-α）的分泌。此外，PEI-PSP-CaCO 诱导引流淋巴结（dLNs）中树突状细胞（DCs）、T 细胞和生发中心（GC）B 细胞的激活。它还增强了淋巴细胞增殖，增加了 CD4/CD8 T 细胞的比例，并提高了脾淋巴细胞中 CD3 CD69 T 细胞的频率。因此，PEI-PSP-CaCO 微粒诱导了更强的细胞和体液免疫应答，可作为疫苗递送和佐剂系统潜在应用。
