University of Tasmania, Hobart, TAS, Australia.
Monash University, Melbourne, VIC, Australia.
Methods Mol Biol. 2024;2826:151-163. doi: 10.1007/978-1-0716-3950-4_12.
Intracellular flow cytometry is a powerful technique that can be used to interrogate signalling in rare cellular populations. The strengths of the technique are that massively parallel readouts can be gained from thousands of single cells simultaneously, and the assay is fast and relatively straightforward. This plate-based protocol enables different doses and different timepoints of stimulation to be assessed and has been optimized for rare B cell populations. Combining this technique with high-dimensional flow cytometry enables multiple signalling proteins to be measured with high confidence.
细胞内流式细胞术是一种强大的技术，可用于研究稀有细胞群体中的信号转导。该技术的优势在于可以同时从数千个单细胞中获得大量平行的读数，并且该测定快速且相对简单。该平板测定法可评估不同剂量和不同刺激时间点，并已针对稀有 B 细胞群体进行了优化。将该技术与高维流式细胞术相结合，可以高度可信地测量多种信号蛋白。
