将鼠源性杂交瘤转化为塔斯马尼亚恶魔重组 IgG 抗体。
Conversion of Mouse-Derived Hybridomas to Tasmanian Devil Recombinant IgG Antibodies.
机构信息
Menzies Institute for Medical Research, College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia.
出版信息
Methods Mol Biol. 2024;2826:231-249. doi: 10.1007/978-1-0716-3950-4_17.
The hybridoma method for production of monoclonal antibodies has been a cornerstone of biomedical research for several decades. Here we convert the monoclonal antibody sequence from mouse-derived hybridomas into a "devilized" recombinant antibody with devil IgG heavy chain and IgK light chain. The chimeric recombinant antibody can be used in functional assays, immunotherapy, and to improve understanding of antibodies and Fc receptors in Tasmanian devils. The process can be readily modified for other species.
杂交瘤方法生产单克隆抗体已经是几十年来生物医学研究的基石。在这里，我们将来源于鼠源杂交瘤的单克隆抗体序列改造成具有恶魔 IgG 重链和 IgK 轻链的“恶魔化”重组抗体。嵌合重组抗体可用于功能测定、免疫治疗，并加深对塔斯马尼亚恶魔抗体和 Fc 受体的理解。该过程可方便地修改用于其他物种。
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