de Almeida Lucas Henrique, Gonçalves Maísa de Carvalho, da Conceição Bispo Pitágoras
Laboratório de Biologia Aquática, Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista, Assis, São Paulo, Brazil.
PPG em Entomologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.
PLoS One. 2024 Jul 17;19(7):e0305824. doi: 10.1371/journal.pone.0305824. eCollection 2024.
Kempnyia (Plecoptera: Perlidae) is an endemic genus of Brazilian stoneflies that has 36 valid species and is distributed primarily in the Atlantic Forest and the mountainous areas of Central Brazil, particularly in Goiás and Tocantins states. Despite being the Brazilian genus with the most DNA sequences available on GenBank, integrative studies on the genus began only recently, in 2014. In this context, herein we studied the morphology and molecular data of Kempnyia specimens deposited in the Aquatic Biology Laboratory (UNESP, Assis) and the Entomology Museum of the Federal University of Viçosa (UFVB, Viçosa) collections. For the integrative approach adopted, in addition to studying the specimens morphologically, we used sequences of the COI mitochondrial gene combined with the following species delimitation methods: Automatic Barcode Gap Discovery (ABGD), both primary (ABGDp) and recursive (ABGDr) partitions; Assemble Species by Automatic Partitioning (ASAP); Poisson Tree Processes (PTP) and the Bayesian implementation of the Poisson Tree Processes (bPTP). As a result, we provided 28 new COI sequences of 21 species and support the description of four new species, namely, K. guarani sp. nov., K. tupiniquim sp. nov., K. una sp. nov., and K. zwickii sp. nov., consequently increasing the known diversity of the genus to 40 species. We also discuss the morphological variations observed in other species of the genus and provide several new geographic records. Therefore, our study brings new insights into the values of intra- and interspecific molecular divergence within Kempnyia, serving as a basis for new studies.
肯普石蝇属（襀翅目：齿蛉科）是巴西石蝇的一个特有属，有36个有效物种，主要分布在大西洋森林和巴西中部山区，特别是在戈亚斯州和托坎廷斯州。尽管它是在GenBank上可获得DNA序列最多的巴西属，但对该属的综合研究直到2014年才刚刚开始。在此背景下，我们研究了保存在水生生物学实验室（圣保罗州立大学，阿西斯）和维索萨联邦大学昆虫博物馆（维索萨联邦大学，维索萨）馆藏中的肯普石蝇属标本的形态和分子数据。对于所采用的综合方法，除了对标本进行形态学研究外，我们还使用了细胞色素氧化酶亚基I（COI）线粒体基因序列，并结合以下物种界定方法：自动条形码间隙发现法（ABGD），包括初级（ABGDp）和递归（ABGDr）划分；自动划分组装物种法（ASAP）；泊松树过程法（PTP）以及泊松树过程法的贝叶斯实现（bPTP）。结果，我们提供了21个物种的28条新的COI序列，并支持描述四个新物种，即瓜拉尼肯普石蝇（K. guarani sp. nov.）、图皮尼克姆肯普石蝇（K. tupiniquim sp. nov.）、乌纳肯普石蝇（K. una sp. nov.）和茨维基肯普石蝇（K. zwickii sp. nov.），从而使该属已知的多样性增加到40个物种。我们还讨论了在该属其他物种中观察到的形态变异，并提供了几个新的地理记录。因此，我们的研究为肯普石蝇属种内和种间分子差异的价值带来了新的见解，为新的研究奠定了基础。
