Liu Wuyun, Yu Huarong, Gurbazar D, Rinchindorj D, Kang Wei, Qi Chelimuge, Chen Hongsong, Chang Xu, You Huan, Han Yongmei, Li Zhigang, R G Ahmed, Dong Wu
Key Laboratory of Ecological Agriculture in Horqin Sandy Land, State Ethnic Affairs Commission, Wuhan, China.
College of Agriculture, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China.
PLoS One. 2024 Jul 17;19(7):e0307269. doi: 10.1371/journal.pone.0307269. eCollection 2024.
Urtica cannabina L. (UL) has been used clinically for centuries because of its anti-inflammatory properties. This study aimed to investigate the underlying mechanisms and anti-inflammatory effects of different UL concentrations in zebrafish. To elucidate UL's anti-inflammatory properties, two inflammation zebrafish models were designed 1) by severing the zebrafish's caudal fin to assess the repairing effect of UL on the tail inflammation, and 2) by inducing lipopolysaccharides (LPS)-mediated intestinal inflammation to assess the protective and reparative effects of UL on intestinal inflammation at the histological and genetic levels. Furthermore, the effect of UL on the LPS-induced intestinal flora changes was also assessed. After caudal fin resection, a scar formed on the tail of the zebrafish, and the area of the caudal fin increased by 1.30 times as much as that of the control group (P < 0.01). Moreover, this tail scar was alleviated after 10 mg/g UL supplementation but not after 30 mg/g UL dose. LPS decreased the feed intake and body weight of the zebrafish; however, these effects were reversed after 10 and 30 mg/g doses of UL. In addition, the LPS treatment also reduced the intestinal goblet cells by 49% in the zebrafish when compared with the control, which was significantly restored after 10 and 30 mg/g UL treatments. At the genetics level, the expression of the pro-inflammatory cytokine genes (TNF-α, IL6, and IL8) showed that 10 and 30 mg/g UL doses could rescue LPS-induced expression. The gut microbiota analysis revealed changes in the abundance of four major bacterial phyla in the 10 and 30 mg/g UL-treated groups, with an increased probiotic Bacteroidota and decreased pathogenic bacteria. These results indicate that UL strongly inhibits inflammation caused by caudal fin removal and LPS-induced inflammatory changes in the zebrafish intensity, suggesting that UL is a feed additive that could be developed to improve resistance to inflammation in livestock.
大麻叶荨麻（Urtica cannabina L.，UL）因其抗炎特性已在临床上使用了数百年。本研究旨在探讨不同浓度的UL在斑马鱼体内的潜在作用机制和抗炎效果。为阐明UL的抗炎特性，设计了两种炎症斑马鱼模型：1）通过切断斑马鱼的尾鳍来评估UL对尾部炎症的修复作用；2）通过诱导脂多糖（LPS）介导的肠道炎症，从组织学和基因水平评估UL对肠道炎症的保护和修复作用。此外，还评估了UL对LPS诱导的肠道菌群变化的影响。尾鳍切除后，斑马鱼尾部形成疤痕，尾鳍面积比对照组增加了1.30倍（P < 0.01）。此外，补充10 mg/g UL后尾部疤痕减轻，但30 mg/g UL剂量下未减轻。LPS降低了斑马鱼的采食量和体重；然而，10和30 mg/g剂量的UL可逆转这些影响。此外，与对照组相比，LPS处理还使斑马鱼肠道杯状细胞减少了49%，10和30 mg/g UL处理后显著恢复。在基因水平上，促炎细胞因子基因（TNF-α、IL6和IL8）的表达表明，10和30 mg/g UL剂量可挽救LPS诱导的表达。肠道微生物群分析显示，10和30 mg/g UL处理组中四个主要细菌门的丰度发生了变化，益生菌拟杆菌门增加，病原菌减少。这些结果表明，UL强烈抑制尾鳍切除和LPS诱导的斑马鱼炎症变化，表明UL是一种可开发用于提高家畜抗炎症能力的饲料添加剂。
