College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Bluestar Adisseo Nanjing Co. Ltd., Nanjing, 210000, China.
J Anim Sci. 2021 Nov 1;99(11). doi: 10.1093/jas/skab296.
Bisdemethoxycurcumin has good antioxidant and anti-inflammatory effects and has been widely used as food and feed supplements in the form of curcuminoids. However, the beneficial effect of individual bisdemethoxycurcumin on preventing lipopolysaccharide (LPS)-induced inflamed intestinal damage is unclear. The present study aimed to investigate whether dietary bisdemethoxycurcumin supplementation could attenuate LPS-induced intestinal damage and alteration of cecal microbiota in broiler chickens. In total, 320 one-day-old male Arbor Acres broiler chickens with a similar weight were randomly divided into four treatments. The treatments were designed as a 2 × 2 factorial arrangement: basal diet (CON); 150 mg/kg bisdemethoxycurcumin diet (BUR); LPS challenge + basal diet (LPS); LPS challenge + 150 mg/kg bisdemethoxycurcumin diet (L-BUR). Results showed that dietary bisdemethoxycurcumin supplementation attenuated the LPS-induced decrease of average daily feed intake. LPS challenge compromised the intestinal morphology and disrupted the intestinal tight junction barrier. Dietary bisdemethoxycurcumin supplementation significantly increased villus length:crypt depth ratio and upregulated the mRNA expression of intestinal tight junction proteins. Moreover, a remarkably reduced mRNA expression of inflammatory mediators was observed following bisdemethoxycurcumin supplementation. The cecal microbiota analysis showed that bisdemethoxycurcumin supplementation increased the relative abundance of the genus Faecalibacterium while decreased the relative abundance of the genera Bacteroides and Subdoligranulum. In conclusion, dietary bisdemethoxycurcumin supplementation could counteract LPS-induced inflamed intestinal damage in broiler chickens by improving intestinal morphology, maintaining intestinal tight junction, downregulating pro-inflammatory mediators, and restoring cecal microbiota.
双去甲氧基姜黄素具有良好的抗氧化和抗炎作用，已作为姜黄素类化合物以食品和饲料添加剂的形式广泛应用。然而，双去甲氧基姜黄素对预防脂多糖（LPS）诱导的炎症性肠损伤的有益作用尚不清楚。本研究旨在探讨饲粮补充双去甲氧基姜黄素是否能减轻 LPS 诱导的肉鸡肠道损伤和盲肠微生物群的改变。总共将 320 只 1 日龄、体重相近的艾维茵肉鸡随机分为 4 个处理组，采用 2×2 因子设计：基础日粮（CON）；150mg/kg 双去甲氧基姜黄素日粮（BUR）；LPS 挑战+基础日粮（LPS）；LPS 挑战+150mg/kg 双去甲氧基姜黄素日粮（L-BUR）。结果表明，饲粮补充双去甲氧基姜黄素可减轻 LPS 诱导的平均日采食量下降。LPS 挑战破坏了肠道形态，破坏了肠道紧密连接屏障。饲粮补充双去甲氧基姜黄素显著增加了绒毛长度：隐窝深度比值，并上调了肠道紧密连接蛋白的 mRNA 表达。此外，双去甲氧基姜黄素补充还显著降低了炎症介质的 mRNA 表达。盲肠微生物群分析表明，双去甲氧基姜黄素补充增加了粪杆菌属的相对丰度，而降低了拟杆菌属和 Subdoligranulum 属的相对丰度。综上所述，饲粮补充双去甲氧基姜黄素可通过改善肠道形态、维持肠道紧密连接、下调促炎介质和恢复盲肠微生物群来抵抗 LPS 诱导的肉鸡肠道炎症损伤。
