Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Bioorg Med Chem. 2024 Aug 1;110:117838. doi: 10.1016/j.bmc.2024.117838. Epub 2024 Jul 14.
Prenylation (isopentenylation), a key post-transcriptional modification with a hydrophobic prenyl group onto the biomacromolecules such as RNA and proteins, influences their localization and function. Prenyltransferases mediate this process, while cytokinin oxidases degrade the prenylated adenosine in plants. This review summarizes current progress in detecting prenylation modifications in RNA across species and their effects on protein synthesis. Advanced methods have been developed to label and study these modifications in vitro and in vivo, despite challenges posed by the inert chemical properties of prenyl groups. Continued advancements in bioorthogonal chemistry promise new tools for understanding the precise biological functions of prenylated RNA modifications and other related proteins.
prenylation（异戊烯基化），一种关键的转录后修饰，将疏水性的异戊烯基基团连接到生物大分子如 RNA 和蛋白质上，影响它们的定位和功能。prenyltransferases 介导这一过程，而细胞分裂素氧化酶在植物中降解被prenylated 的腺苷。本综述总结了目前在不同物种中检测 RNA prenylation 修饰及其对蛋白质合成影响的进展。尽管 prenyl 基团的惰性化学性质带来了挑战，但已经开发出了先进的方法来标记和研究这些修饰在体外和体内的情况。生物正交化学的持续进步有望为理解 prenylated RNA 修饰和其他相关蛋白质的精确生物学功能提供新的工具。
