Department of Hand and Foot Microsurgery, The affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang 421002, China; Medical School, University of Chinese Academy of Sciences, Beijing 100010, China; Department of Rheumatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Clinical Immunology Center, Beijing 100010, China.
Department of Hand and Foot Microsurgery, The affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang 421002, China.
Colloids Surf B Biointerfaces. 2024 Oct;242:114095. doi: 10.1016/j.colsurfb.2024.114095. Epub 2024 Jul 14.
Bacterial infections and the degeneration of the capillary network comprise the primary factors that contribute to the delayed healing of diabetic wounds. However, treatment modalities that cater to effective diabetic wounds healing in clinical settings are severely lacking. Herein, a dual-functional microsphere carrier was designed, which encapsulates polyhexamethylene biguanide (PHMB) or recombinant human vascular endothelial growth factor (rhVEGF) together. The in vitro release experiments demonstrated that the use of the microspheres ensured the sustained release of the drugs (PHMB or rhVEGF) over a period of 12 days. Additionally, the integration of these controlled-release microspheres into a dermal scaffold (DS-PLGA@PHMB/rhVEGF) imbued both antibacterial and angiogenic functions to the resulting material. Accordingly, the DS-PLGA@PHMB/rhVEGF scaffold exhibited potent antibacterial properties, effectively suppressing bacterial growth and providing a conducive environment for wound healing, thereby addressing the drawbacks associated with the susceptibility of rhVEGF to deactivation in inflammatory conditions. Additionally, the histological analysis revealed that the use of the DS-PLGA@PHMB/rhVEGF scaffold accelerated the process of wound healing by inhibiting inflammatory reactions, stimulating the production of collagen formation, and enhancing angiogenesis. This provides a novel solution for enhancing the antibacterial and vascularization capabilities of artificial dermal scaffolds, providing a beacon of hope for improving diabetic wound healing.
细菌感染和毛细血管网络退化是导致糖尿病伤口愈合延迟的主要因素。然而，临床上缺乏有效的糖尿病伤口愈合治疗方法。在此，设计了一种具有双重功能的微球载体，将聚六亚甲基双胍（PHMB）或重组人血管内皮生长因子（rhVEGF）同时包封在其中。体外释放实验表明，微球的使用可确保药物（PHMB 或 rhVEGF）在 12 天内持续释放。此外，将这些控释微球整合到真皮支架（DS-PLGA@PHMB/rhVEGF）中，赋予了材料抗菌和血管生成功能。因此，DS-PLGA@PHMB/rhVEGF 支架具有强大的抗菌性能，有效抑制细菌生长，为伤口愈合提供有利环境，从而解决了 rhVEGF 在炎症条件下易失活的缺点。此外，组织学分析表明，使用 DS-PLGA@PHMB/rhVEGF 支架通过抑制炎症反应、刺激胶原蛋白形成和促进血管生成来加速伤口愈合过程。这为增强人工真皮支架的抗菌和血管生成能力提供了一种新的解决方案，为改善糖尿病伤口愈合带来了希望。
