CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China; College of Agriculture and Biological Sciences, Dali University, Dali 671003, China.
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China.
Microbiol Res. 2024 Sep;286:127829. doi: 10.1016/j.micres.2024.127829. Epub 2024 Jul 14.
The impact of climate warming on soil microbes has been well documented, with studies revealing its effects on diversity, community structure and network dynamics. However, the consistency of soil microbial community assembly, particularly in response to diverse plant root exudates under varying temperature conditions, remains an unresolved issue. To address this issue, we employed a growth chamber to integrate temperature and root exudates in a controlled experiment to examine the response of soil bacteria, fungi, and protists. Our findings revealed that temperature independently regulated microbial diversity, with distinct patterns observed among bacteria, fungi, and protists. Both root exudates and temperature significantly influenced microbial community composition, yet interpretations of these factors varied among prokaryotes and eukaryotes. In addition to phototrophic bacteria and protists, as well as protistan consumers, root exudates determined to varying degrees the enrichment of other microbial functional guilds at specific temperatures. The effects of temperature and root exudates on microbial co-occurrence patterns were interdependent; root exudates primarily simplified the network at low and high temperatures, while responses to temperature varied between single and mixed exudate treatments. Moreover, temperature altered the composition of keystone species within the microbial network, while root exudates led to a decrease in their number. These results emphasize the substantial impact of plant root exudates on soil microbial community responses to temperature, underscoring the necessity for future climate change research to incorporate additional environmental variables.
气候变暖对土壤微生物的影响已有大量记录，研究揭示了其对多样性、群落结构和网络动态的影响。然而，土壤微生物群落组装的一致性，特别是在不同温度条件下对不同植物根系分泌物的响应，仍然是一个未解决的问题。为了解决这个问题，我们使用生长室将温度和根系分泌物整合在一个控制实验中，以研究土壤细菌、真菌和原生动物的反应。我们的研究结果表明，温度独立地调节微生物多样性，细菌、真菌和原生动物之间存在明显的模式。根系分泌物和温度都显著影响微生物群落组成，但这些因素在原核生物和真核生物中的解释不同。除了光合细菌和原生动物以及原生动物消费者外，根系分泌物还在不同程度上决定了其他微生物功能类群在特定温度下的富集。温度和根系分泌物对微生物共现模式的影响是相互依存的；根系分泌物主要在低温和高温下简化网络，而对温度的响应在单一和混合分泌物处理之间存在差异。此外，温度改变了微生物网络中关键种的组成，而根系分泌物导致其数量减少。这些结果强调了植物根系分泌物对土壤微生物群落对温度响应的重大影响，突出了未来气候变化研究必须纳入其他环境变量的必要性。
