School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, China.
School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, China.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133915. doi: 10.1016/j.ijbiomac.2024.133915. Epub 2024 Jul 15.
The low solubility of chitosan (CS) imposes adverse effects on its application. In this work, one of the aims is to improve the water solubility of CS. By introducing water-soluble side chains to CS, this aim was achieved. Besides, fluorescent moieties were incorporated into the side chains, the fluorescent copolymers were endowed with Cr and Cu ions recognition ability. Firstly, a reversible addition-fragmentation chain transfer polymerization (RAFT) reagent with naphthalimide units and CC groups was prepared. Water-soluble monomer methyl acrylic acid (MAA) was employed in the RAFT polymerization. Thus, water-soluble polymer with fluorescent unit and -C ≡ C on both ends of the polymer was obtained. They were introduced into CS, and the CS-based fluorescent copolymers were obtained eventually. The amount of MAA introduced could be tuned to obtain three side chains of different lengths. It was found that the more MAA was introduced, the better the solubility of CS-TP was. The detection limits (LOD) of Cr and Cu were 44.6 nM and 54.5 nM, respectively. The detection of Cr and Cu ions is further combined with a mobile APP to realize real-time, portable, and visual detection. And the application in the logic gate, a new detection platform, is prepared.
壳聚糖（CS）的低溶解度对其应用有不利影响。在这项工作中，目的之一是提高 CS 的水溶性。通过向 CS 中引入水溶性侧链，实现了这一目的。此外，荧光部分被引入侧链，赋予荧光共聚物对 Cr 和 Cu 离子的识别能力。首先，制备了带有萘酰亚胺单元和 CC 基团的可逆加成-断裂链转移聚合（RAFT）试剂。在 RAFT 聚合中使用了水溶性单体甲基丙烯酸（MAA）。因此，得到了在聚合物两端具有荧光单元和 -C≡C 的水溶性聚合物。将它们引入 CS 中，最终得到了基于 CS 的荧光共聚物。可以调节引入的 MAA 的量，以获得三种不同长度的侧链。结果发现，引入的 MAA 越多，CS-TP 的溶解度越好。Cr 和 Cu 的检测限（LOD）分别为 44.6 nM 和 54.5 nM。Cr 和 Cu 离子的检测进一步与移动 APP 相结合，实现了实时、便携和可视化检测。并制备了用于逻辑门的新检测平台。
