Hlača Nika, Vičić Marijana, Kaštelan Marija, Dekanić Andrea, Prpić-Massari Larisa
Department of Dermatovenerology, Faculty of Medicine, University of Rijeka, Clinical Hospital Center Rijeka, Krešimirova 42, 51000, Rijeka, Croatia.
Department of Pathology, Faculty of Medicine, University of Rijeka, Clinical Hospital Center Rijeka, Krešimirova 42, 51000, Rijeka, Croatia.
Sci Rep. 2024 Jul 17;14(1):16580. doi: 10.1038/s41598-024-67494-9.
Vitiligo and halo nevus are immune-mediated skin diseases that have a similar pathogenesis and involve cellular cytotoxicity mechanisms that are not yet fully understood. In this study, we investigated the expression patterns of the cytolytic molecule granulysin (GNLY) in different cytotoxic cells in skin samples of vitiligo and halo nevus. Skin biopsies were taken from perilesional and lesional skin of ten vitiligo patients, eight patients with halo nevus and ten healthy controls. We analysed the expression of GNLY by immunohistochemistry in CD8+ and CD56+ NK cells. A significantly higher accumulation of GNLY+, CD8+ GNLY+ and fewer CD56+ GNLY+ cells was found in the lesional skin of vitiligo and halo nevus than in the healthy skin. These cells were localised in the basal epidermis and papillary dermis, suggesting that GNLY may be involved in the immune response against melanocytes. Similarly, but to a lesser extent, upregulation of GNLY+ and CD8+ GNLY+ cells was observed in the perilesional skin of vitiligo and halo nevus compared to healthy controls. In this study, we demonstrated for the first time an increased expression of CD8+ GNLY+ T lymphocytes and CD56+ GNLY+ NK cells in lesions of vitiligo and halo nevus, indicating the role of GNLY in the pathogenesis of both diseases.
白癜风和晕痣是免疫介导的皮肤病，它们具有相似的发病机制，涉及尚未完全了解的细胞毒性机制。在本研究中，我们调查了细胞溶解分子颗粒溶素（GNLY）在白癜风和晕痣皮肤样本中不同细胞毒性细胞中的表达模式。从10例白癜风患者、8例晕痣患者和10名健康对照者的皮损周围和皮损处皮肤进行活检。我们通过免疫组织化学分析了GNLY在CD8 +和CD56 +自然杀伤细胞中的表达。在白癜风和晕痣的皮损皮肤中发现GNLY +、CD8 + GNLY +细胞的积累明显高于健康皮肤，而CD56 + GNLY +细胞较少。这些细胞位于基底表皮和乳头真皮，提示GNLY可能参与针对黑素细胞的免疫反应。同样，与健康对照相比，在白癜风和晕痣的皮损周围皮肤中观察到GNLY +和CD8 + GNLY +细胞的上调，但程度较轻。在本研究中，我们首次证明白癜风和晕痣皮损中CD8 + GNLY + T淋巴细胞和CD56 + GNLY +自然杀伤细胞表达增加，表明GNLY在这两种疾病发病机制中的作用。
