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将菠萝蛋白酶固定在粘土-羧甲基纤维素复合材料上以提高豆粕营养价值。

Bromelain Immobilized onto Clay-Carboxymethylcellulose Composites for Improving Nutritive Value of Soybean Meal.

机构信息

School of Integrated Science and Innovation, Sirindhorn International Institute of Technology (SIIT), Thammasat University, Pathum Thani 12121, Thailand.

National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand.

出版信息

ACS Appl Bio Mater. 2024 Aug 19;7(8):5211-5221. doi: 10.1021/acsabm.4c00392. Epub 2024 Jul 18.

DOI:10.1021/acsabm.4c00392
PMID:39021071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11337166/
Abstract

Improvement of nutritional value and reduction of antinutritional factors (ANFs) of soybean meal (SBM) for animal feed applications could be achieved by using bromelain immobilized onto bentonite (Bt)-carboxymethylcellulose (CMC) composites. The composite with mass ratio between CMC to calcium ion (Ca) at 1:20 provided the highest enzyme activity, immobilization yield higher than 95%, with superior thermal and storage stabilities. Performance of the immobilized bromelain for soybean protein hydrolysis was further studied. The results showed that at 60 °C, the immobilized bromelain exhibited the highest efficiency in enzymatic hydrolysis to release free alpha amino nitrogen (FAN) as a product with high selectivity and to effectively reduce SBM allergenic proteins within 30 min. In conclusion, immobilization of bromelain onto Bt-CMC composites leads to stability enhancement of the enzyme, enabling effective improvement in SBM quality in a short treatment time and showing great potential for application in animal feed industries.

摘要

通过将菠萝蛋白酶固定在膨润土（Bt）-羧甲基纤维素（CMC）复合材料上，可以提高用于动物饲料应用的大豆蛋白粉（SBM）的营养价值并降低抗营养因子（ANFs）。当 CMC 与钙离子（Ca）的质量比为 1:20 时，复合材料提供了最高的酶活性，固定化收率高于 95%，具有优异的热稳定性和储存稳定性。进一步研究了固定化菠萝蛋白酶对大豆蛋白水解的性能。结果表明，在 60°C 下，固定化菠萝蛋白酶在酶解释放游离α-氨基酸氮（FAN）作为产物方面表现出最高的效率，具有高选择性，并能在 30 分钟内有效降低 SBM 过敏原蛋白。总之，将菠萝蛋白酶固定在 Bt-CMC 复合材料上可提高酶的稳定性，可在短时间内有效改善 SBM 的质量，并在动物饲料工业中有很大的应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/861e456f9cbc/mt4c00392_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/f7658c853b1c/mt4c00392_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/0d47e7547c26/mt4c00392_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/72e12a7909cc/mt4c00392_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/2cbb35439428/mt4c00392_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/357df7404af0/mt4c00392_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/4682161ce33c/mt4c00392_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/42dc0f95cc49/mt4c00392_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/8fdd334adf74/mt4c00392_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/ff7cb6d270b6/mt4c00392_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/861e456f9cbc/mt4c00392_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/f7658c853b1c/mt4c00392_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/0d47e7547c26/mt4c00392_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/72e12a7909cc/mt4c00392_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/2cbb35439428/mt4c00392_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/357df7404af0/mt4c00392_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/4682161ce33c/mt4c00392_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/42dc0f95cc49/mt4c00392_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/8fdd334adf74/mt4c00392_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/ff7cb6d270b6/mt4c00392_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b138/11337166/861e456f9cbc/mt4c00392_0010.jpg

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