Kadhem Ali Hakem, Gholizadeh Ashraf
Division of Biochemistry, Faculty of Natural Sciences, University of Tabriz Tabriz, Iran.
Division of Biochemistry, Faculty of Natural Sciences and Research Center for Bioscience and Biotechnology (RCBB), University of Tabriz Tabriz, Iran.
Int J Biochem Mol Biol. 2024 Jun 15;15(3):60-67. doi: 10.62347/XARB9847. eCollection 2024.
The current study aimed to correlate OCT1 (organic cation transporter 1) polymorphisms with metformin response variability in Iraqi women with PCOS (polycystic ovarian syndrome) and determine the impact of OCT1 polymorphism. PCOS, an endocrine metabolic disorder, can seriously impact female health including infertility. Although its cause is unclear, it is usually known to be associated with hormonal imbalances. OCT1 is essential for metformin absorption in the liver. Recent research shown that OCT1 polymorphisms affects metformin responsiveness.
In the present work, a prospective case-control study was conducted at Department of Infertility, Karbala Teaching Hospital for Obstetrics and Gynecology. 100 PCOS patients and 50 healthy controls aged 20-40 were enrolled. Consultant gynecologist diagnosed PCOS patients using Rotterdam criteria and recommended metformin 500 mg twice daily for 3 months. At the start of the trial and after 3 months, all patients and healthy controls underwent hormonal, biochemical and genetic tests.
The similar allelic frequencies of OCT1 polymorphism in PCOS and control groups was observed. Most patients with reference wild type alleles (C) showed considerable hormonal and metabolic responses to metformin, while those with mutant alleles (T) showed non-significant responses.
FSH, prolactin and testosterone hormonal levels may be considered as candidate biomarkers for PCOS detection and metformin related biomedical respond.
本研究旨在探讨伊拉克多囊卵巢综合征（PCOS）女性中有机阳离子转运体1（OCT1）基因多态性与二甲双胍反应变异性之间的相关性，并确定OCT1基因多态性的影响。PCOS是一种内分泌代谢紊乱疾病，会严重影响女性健康，包括不孕。虽然其病因尚不清楚，但通常认为与激素失衡有关。OCT1对肝脏中二甲双胍的吸收至关重要。最近的研究表明，OCT1基因多态性会影响二甲双胍的反应性。
在卡尔巴拉妇产科教学医院不孕症科进行了一项前瞻性病例对照研究。纳入了100例年龄在20至40岁之间的PCOS患者和50例健康对照者。妇科顾问医生使用鹿特丹标准诊断PCOS患者，并建议每天两次服用500毫克二甲双胍，持续3个月。在试验开始时和3个月后，所有患者和健康对照者都接受了激素、生化和基因检测。
观察到PCOS组和对照组中OCT1基因多态性的等位基因频率相似。大多数具有参考野生型等位基因（C）的患者对二甲双胍表现出显著的激素和代谢反应，而具有突变等位基因（T）的患者则表现出不显著的反应。
促卵泡生成素、催乳素和睾酮激素水平可被视为PCOS检测和二甲双胍相关生物医学反应的候选生物标志物。
