Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China.
Center for Laboratory Animal Research of Xinjiang, Center for Disease Control of Xinjiang, No. 138, Jianquan Yi Jie, Tianshan District, Urumqi City, Xinjiang Uygur Autonomous Region, 830011, P.R. China.
Exp Anim. 2020 Apr 24;69(2):224-232. doi: 10.1538/expanim.19-0077. Epub 2020 Feb 14.
Understanding the genetic quality of the gerbil, Meriones meridianus, plays an important role in the study of medical biology. However, no effective system has been established for evaluating a population's genetic diversity to date. In the present study, we established a set of reasonable evaluative systems based on microsatellite markers of the Mongolian gerbil by using the method of cross-amplification of species. Following electrophoresis analysis, short tandem repeat (STR) scanning, and sequencing, 11 microsatellite loci were identified by matching the criteria characteristics and were used to evaluate the genetic diversity of two stocks of Meriones meridianus: Meriones meridianus jei Wang, 1964 (M. m. jei) and Meriones meridianus cryptorhinus Blanford, 1875 (M. m. cryptorhinus) from Xinjiang, China. The microsatellite loci screened were highly polymorphic and were suitable for genetic quality control of Meriones meridianus. In addition, the quality of the non-bred M. m. jei and M. m. cryptorhinus strains in our study is sufficient for them to be promising stocks in the future for the farmed animal industry.
了解子午沙鼠（Meriones meridianus）的遗传质量在医学生物学研究中起着重要作用。然而，迄今为止，尚未建立有效的系统来评估种群的遗传多样性。在本研究中，我们通过物种交叉扩增的方法，利用蒙古沙鼠的微卫星标记，建立了一套合理的评价体系。通过电泳分析、短串联重复（STR）扫描和测序，根据特征匹配标准，确定了 11 个微卫星位点，并用于评估来自中国新疆的两个子午沙鼠种群的遗传多样性：黑线仓鼠指名亚种（Meriones meridianus jei Wang, 1964，M. m. jei）和黑线仓鼠新疆亚种（Meriones meridianus cryptorhinus Blanford, 1875，M. m. cryptorhinus）。筛选出的微卫星位点高度多态，适合子午沙鼠的遗传质量控制。此外，本研究中非繁殖的 M. m. jei 和 M. m. cryptorhinus 品系的质量足以保证它们在未来成为有前途的养殖动物种群。
