Ramos Rafael A N, Hakimi Hassan, Salomon Jordan, Busselman Rachel E, Curtis-Robles Rachel, Hodo Carolyn L, Hamer Sarah A, Verocai Guilherme G
Department of Veterinary Pathobiology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Laboratory of Parasitology, Federal University of the Agreste of Pernambuco, Garanhuns, PE, 55282-278, Brazil.
Int J Parasitol Parasites Wildl. 2024 Jun 12;24:100954. doi: 10.1016/j.ijppaw.2024.100954. eCollection 2024 Aug.
and (Spirurida: Onchocercidae) are epidemiologically important filarial nematodes detected in wild carnivores sympatric to domestic animals and humans. In this study we surveyed for species among previous studies archived blood samples (n = 202) of wild carnivores sourced across Texas between the years of 2014-2016 and 2020 to 2023. In total, 117 coyotes (), 67 raccoons (), 12 gray foxes (), five bobcats (), and one striped skunk () were tested through the amplification of the partial cytochrome oxidase c subunit 1 () gene followed by sequencing. was detected in 11.39% (95% CI = 7.71-16.51) of the samples (21 coyotes and two raccoons), while was detected in a single bobcat. sequences had 99.85%-100% (99.92% ± 0.08) similarity with other sequences in GenBank. The sequence of from the bobcat was 100% similar to the single sequence available in GenBank. Data from this study reinforce the role of coyotes as a wild reservoir for and suggest that raccoons may also play a role in the epidemiology of this parasite. This study additionally provides molecular data on , an understudied filarioid of felids.
（旋尾目：盘尾丝虫科）是在与家畜和人类同域分布的野生食肉动物中检测到的具有重要流行病学意义的丝虫线虫。在本研究中，我们在2014 - 2016年以及2020年至2023年期间从得克萨斯州采集的野生食肉动物存档血液样本（n = 202）中调查了 物种。总共对117只郊狼（ ）、67只浣熊（ ）、12只灰狐（ ）、5只山猫（ ）和1只条纹臭鼬（ ）进行了检测，方法是扩增细胞色素氧化酶c亚基1（ ）部分基因，然后进行测序。在11.39%（95%置信区间 = 7.71 - 16.51）的样本（21只郊狼和2只浣熊）中检测到了 ，而在1只山猫中检测到了 。 序列与GenBank中其他 序列的相似度为99.85% - 100%（99.92% ± 0.08）。来自山猫的 序列与GenBank中唯一可用的 序列100%相似。本研究的数据强化了郊狼作为 的野生宿主的作用，并表明浣熊在这种寄生虫的流行病学中可能也发挥作用。本研究还提供了关于 的分子数据， 是一种研究较少的猫科动物丝状线虫。
