Tahir Djamel, Damene Hanane, Davoust Bernard, Parola Philippe
URMITE, Aix Marseille Univ, CNRS, IRD, INSERM, AP-HM, IHU-Méditerranée Infection, Marseille, France.
Institute of Veterinary Sciences, University Blida 1, Blida, Algeria.
Comp Immunol Microbiol Infect Dis. 2017 Apr;51:66-68. doi: 10.1016/j.cimid.2017.04.001. Epub 2017 Apr 14.
Dirofilaria immitis and Dirofilaria repens are mosquito-borne filarioid nematodes that affect dogs and other domestic and wild carnivores, causing heartworm disease and subcutaneous dirofilariosis, respectively. In Algeria, the data about the epidemiology of these infections is largely unknown. The present study was designed to establish the occurrence of D. immitis and D. repens in dogs in Algeria using molecular tools. In 2014 and 2015, a total of 209 dogs over one year of age of different breed and sex, living in Northern Algeria, were examined and blood samples were collected from each dog. The presence of D. immitis and D. repens in these samples was detected by real-time PCR followed by standard PCR and sequencing. Overall, the blood of 209 dogs from two departments was collected and only 3 (1.4%) of the blood samples were found positive for D. immitis DNA. Sequencing of the corresponding amplicon displayed a 99.8% identity to D. immitis, confirming the presence of this mosquito-borne nematode in Algeria. Furthermore, all tested samples were negative for D. repens.
犬恶丝虫和匐行恶丝虫是由蚊子传播的类丝虫线虫，分别感染犬类和其他家养及野生食肉动物，引发犬心丝虫病和皮下恶丝虫病。在阿尔及利亚，关于这些感染的流行病学数据很大程度上未知。本研究旨在利用分子工具确定阿尔及利亚犬类中犬恶丝虫和匐行恶丝虫的感染情况。2014年和2015年，对生活在阿尔及利亚北部的209只一岁以上不同品种和性别的犬进行了检查，并从每只犬采集了血样。通过实时PCR，随后进行标准PCR和测序，检测这些样本中犬恶丝虫和匐行恶丝虫的存在情况。总体而言，从两个省份采集了209只犬的血液，仅3份（1.4%）血样检测出犬恶丝虫DNA呈阳性。相应扩增子的测序显示与犬恶丝虫的同一性为99.8%，证实了这种由蚊子传播的线虫在阿尔及利亚的存在。此外，所有检测样本中匐行恶丝虫均为阴性。
