Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, PR China.
Departments of Histology and Embryology, Basic Medical College, Jilin University, Changchun 130021, PR China.
Biosens Bioelectron. 2017 May 15;91:431-435. doi: 10.1016/j.bios.2016.12.058. Epub 2016 Dec 30.
We have developed a sensitive photonic crystal fiber (PCF)-based immunosensor for detection of alpha fetoprotein (AFP). The unique PCF possesses a morphology characterized by numerous pore structures and a large surface area-to-volume ratio, which can be used as an immune-reaction carrier to improve the sensitivity and reaction speed of AFP detection. The PCF-based immunosensor possesses a low limit of detection of 0.1ng/mL, which is five times lower than that of the capillary-based sensor and 35 times lower than that of the traditional enzyme-linked immunosorbent assay. The wide linear dynamic range of 0.1-150ng/mL makes the developed immunosensor suitable for clinical practice. The proposed method was successfully applied to AFP detection in a clinical serum sample with acceptable precision. It is indicated that the present PCF-based immunosensor could be used as an attractive analytical platform for sensitive and specific detection of cancer biomarkers.
我们开发了一种基于光子晶体光纤（PCF）的灵敏免疫传感器，用于检测甲胎蛋白（AFP）。独特的 PCF 具有许多孔结构和大的表面积与体积比的形态，可用作免疫反应载体，以提高 AFP 检测的灵敏度和反应速度。基于 PCF 的免疫传感器具有 0.1ng/mL 的低检测限，比基于毛细管的传感器低五倍，比传统的酶联免疫吸附测定法低 35 倍。0.1-150ng/mL 的宽线性动态范围使开发的免疫传感器适用于临床实践。该方法已成功应用于临床血清样本中 AFP 的检测，具有可接受的精密度。结果表明，本研究中的基于 PCF 的免疫传感器可用作用于灵敏和特异性检测癌症生物标志物的有吸引力的分析平台。
