渗透敏感型酿酒酵母突变体细胞裂解物中的低核糖核酸酶活性
Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants.
作者信息
Venkov P V
出版信息
Mol Gen Genet. 1979 Aug;175(1):111-2. doi: 10.1007/BF00267862.
PMID:390302
Abstract
Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.
摘要
通过在1%山梨醇溶液中裂解酿酒酵母VY1160渗透敏感突变细胞，可获得总核糖核酸酶活性非常低的细胞裂解物。这些裂解物可用于完整多核糖体和信使RNA分子的分离，或用于特定核糖核酸酶的研究。
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