利用一种新型平板分析法寻找脱氧核糖核酸酶缺陷的酵母突变体。
The use of a novel plate assay in a search for yeast mutants defective in deoxyribonucleases.
作者信息
Johnston L H
出版信息
Mol Gen Genet. 1977 Apr 29;152(3):219-22. doi: 10.1007/BF00268821.
PMID:327278
Abstract
Methods by which the intracellular enzymes deoxyribonuclease, ribonuclease and protease can be assayed in whole colonies of Saccharomyces cerevisiae on agar plates are described. A search for mutants deficient in deoxyribonuclease has been carried out. Two types of mutant are described. One apparently fails to produce deoxyribonuclease, ribonuclease or protease on agar plates and the other apparently fails to produce deoxyribonuclease and ribonuclease.
摘要
本文描述了在琼脂平板上对酿酒酵母全菌落中的细胞内酶脱氧核糖核酸酶、核糖核酸酶和蛋白酶进行测定的方法。已开展了对脱氧核糖核酸酶缺陷型突变体的筛选。文中描述了两种类型的突变体。一种在琼脂平板上显然无法产生脱氧核糖核酸酶、核糖核酸酶或蛋白酶，另一种显然无法产生脱氧核糖核酸酶和核糖核酸酶。
Zotero 插件