Hutchison H T, Hartwell L H
J Bacteriol. 1967 Nov;94(5):1697-705. doi: 10.1128/jb.94.5.1697-1705.1967.
Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis in the spheroplasts is nearly the same as that occurring in whole cells incubated under the same conditions; DNA synthesis occurs at about half the whole cell rate. The spheroplasts synthesize transfer RNA and ribosomal RNA. The newly synthesized ribosomal RNA is incorporated into ribosomes and polysomes. The polysomes are the site of protein synthesis in these spheroplasts. Greater than 90% of the total RNA can be solubilized by treatment of the spheroplasts with sodium dodecyl sulfate or sodium deoxycholate. These spheroplast preparations appear to be a useful subject for the study of RNA metabolism in yeast.
已建立了制备酿酒酵母原生质球的条件，这些原生质球在含有1m山梨醇的稳定培养基中孵育时，其蛋白质、核糖核酸（RNA）和脱氧核糖核酸（DNA）的净含量能够增加几倍。原生质球中RNA和蛋白质的合成速率与在相同条件下孵育的完整细胞中的合成速率几乎相同；DNA合成速率约为完整细胞速率的一半。原生质球合成转运RNA和核糖体RNA。新合成的核糖体RNA被整合到核糖体和多聚核糖体中。多聚核糖体是这些原生质球中蛋白质合成的场所。用十二烷基硫酸钠或脱氧胆酸钠处理原生质球，可使总RNA的90%以上溶解。这些原生质体制备物似乎是研究酵母RNA代谢的有用对象。
