Agricultural Microbiology Department, National Research Centre, 33 EL-Buhouth St., Dokki, Cairo, 12622, Egypt.
Protenic and Manmade Fibers Department, National Research Centre, Dokki, Cairo, 12622, Egypt.
Sci Rep. 2024 Jul 19;14(1):16660. doi: 10.1038/s41598-024-66430-1.
The fibrous wastes generated from the mills of textile production can be recycled and converted into high add-values products to be implemented in several applications. The current study aimed to employ commercial free cellulase enzyme to partially hydrolyze (activate) the polyester cotton blended (PET/C) fibrous wastes by creation functional groups such as OH and COOH on their surfaces. The activated fibrous wastes were then modified by coating with ZnO nanoparticles (ZnO-NPs) biosynthesized by actinobacterial cultures free supernatant. The isolate was identified as Streptomyces pseudogriseolus with accession number of OR574241. The conditions that influence the actino-synthesis of ZnO-NPs were optimized and the product was characterized using spectroscopic vision, FTIR, XRD, TEM and SEM. The characteristic ZnO peaks were obviously observed by EDX analysis with 0.38 and 0.75% (wt%), respectively. TEM analyses proved the nanoscale of ZnO-NPs (5-15 nm) which was followed by cytotoxic evaluation for the produced NPs. Fortunately, the tested actino-ZnO-NPs didn't have any cytotoxicity against human normal fibroblast cell line (BJ1), which means that the product can be safely used in a direct-contact with human skin. The treated PET/C blended waste fabrics coated with ZnO-NPs showed high antimicrobial activity and ultraviolet protection values after functionalization by cellulase. EDX analysis demonstrates the presence of Zn peaks on the coated fabrics compared with their absence in blank and control samples, while SEM images showed the formation of a thin layer of ZnO-NPs on the fabric surface. The obtained smart textile can be applied several needed sectors.
纺织生产过程中产生的纤维废料可以回收并转化为高附加值产品，应用于多个领域。本研究旨在利用商业用游离纤维素酶部分水解（激活）涤棉混纺（PET/C）纤维废料，在其表面生成 OH 和 COOH 等官能团。然后，通过用 actinobacterial cultures 游离上清液生物合成的 ZnO 纳米粒子（ZnO-NPs）对活化纤维废料进行涂层改性。分离物被鉴定为 Streptomyces pseudogriseolus，登录号为 OR574241。优化了影响 actino-ZnO-NPs 合成的条件，并通过光谱学、FTIR、XRD、TEM 和 SEM 对产物进行了表征。EDX 分析明显观察到 ZnO 特征峰，分别为 0.38 和 0.75%（wt%）。TEM 分析证明了 ZnO-NPs 的纳米级（5-15nm），随后对所制备的 NPs 进行了细胞毒性评估。幸运的是，测试的 actin-ZnO-NPs 对人正常成纤维细胞系（BJ1）没有任何细胞毒性，这意味着该产品可以安全地与人皮肤直接接触。经纤维素酶处理后，涂覆 ZnO-NPs 的 PET/C 混纺废料织物显示出高抗菌活性和紫外线防护值。EDX 分析表明，与空白和对照样品相比，涂覆织物上存在 Zn 峰，而 SEM 图像显示在织物表面形成了一层 ZnO-NPs 的薄涂层。获得的智能纺织品可应用于多个领域。
