Vespa M N, Lebecq J C, Simonetti N
Istituto di Microbiologia, Facoltà di Farmacia, Università degli Studi di Roma La Sapienzia, Italy.
Mycoses. 1993 Nov-Dec;36(11-12):397-403. doi: 10.1111/j.1439-0507.1993.tb00728.x.
Scanning electron microscopy was used to study the effect of tunicamycin, an inhibitor of N-glycosylation, on the morphology of Candida albicans grown in minimal essential medium Earle's base and supplemented with 0.5% N-acetyl-D-glucosamine to stimulate germ tube induction. Addition of the drug prevented development of germ tubes, development of mycelia from germ tubes and resumption of germ tube growth after drug removal. Inhibited cells increased in size during treatment and subsequently became misshapen and shrivelled with evidence of cytoplasmic spillage.
采用扫描电子显微镜研究衣霉素（一种N-糖基化抑制剂）对在Earle氏基础的最低必需培养基中生长的白色念珠菌形态的影响，该培养基补充有0.5% N-乙酰-D-葡萄糖胺以刺激芽管诱导。添加该药物可阻止芽管的形成、芽管发育成菌丝体以及药物去除后芽管生长的恢复。在处理过程中，受抑制的细胞体积增大，随后变得畸形并萎缩，有细胞质外溢的迹象。
