Nettelblad H, Randolph M A, Weiland A J
J Reconstr Microsurg. 1985 Jan;1(3):177-83. doi: 10.1055/s-2007-1007072.
The capacity of the epiphyseal growth cartilage from the dog fibula to retain its growth potential after heterotopic transfer to a distal radial defect was investigated and compared to orthotopically transferred controls. Autoradiographs of the physes cultured in 3H-thymidine indicated continued proliferation of the growth cartilage in all revascularized transfers at the time of death, six weeks postoperatively. However, the nonvascular orthotopic controls did not incorporate the radioactive label. Fluorescent bone labels were incorporated in parallel bands in the metaphyseal bone, demonstrating linear bone growth in the revascularized transfers. Additional evidence for the viability of the transferred growth cartilage was obtained with histologic sections stained with hematoxylin and eosin and safranin-O. Roentgenograms and volume measurements showed a tendency toward hypertrophy of the heterotopic transfers.
研究了犬腓骨的骺生长软骨异位移植至桡骨远端缺损后保留其生长潜能的能力，并与原位移植的对照进行了比较。在术后六周处死时，对用3H-胸腺嘧啶核苷培养的骺板进行放射自显影，结果表明，在所有血管化移植中，生长软骨在死亡时仍持续增殖。然而，无血管的原位对照未掺入放射性标记。荧光骨标记物在干骺端骨中呈平行带掺入，表明血管化移植中有线性骨生长。用苏木精-伊红和番红O染色的组织学切片获得了移植生长软骨存活的额外证据。X线片和体积测量显示异位移植有肥大的趋势。
