Nettelblad H, Randolph M A, Weiland A J
J Bone Joint Surg Am. 1984 Dec;66(9):1421-30.
To evaluate the feasibility of transplanting vascularized epiphyseal plates while maintaining normal growth in the recipient site, twenty-two puppies from known, large breeds were divided into one control and three experimental groups of four animals each and one long-term group of six animals. The control group underwent insertion of a radiopaque marker in the fibular metaphysis bilaterally, and, in addition, a fibular osteotomy was performed on one side. In the experimental groups, a fibular switch was carried out, selecting one fibula as a vascularized graft and the other as a non-vascularized graft. Both the controls and the experimental groups were evaluated using serial roentgenograms, histological examination, fluorescent bone-labeling, and microangiography. One week, six weeks, three months, and seven months postoperatively, animals from each group were killed. Continuous growth was observed in the vascularized epiphyseal transplants and in the controls, with no statistical difference noted, whereas the non-vascularized transplants exhibited considerably less or no growth. Vascularized transplants demonstrated an average 21.2-millimeter increase in length while non-vascularized transplants showed a 6.6-millimeter increase. Histological examination, fluorochrome bone-labeling, and microangiography confirmed the continued viability of the vascularized epiphyseal transplants in contrast to the non-vascularized transplants.
为评估移植带血管的骺板并使其在受体部位保持正常生长的可行性，将22只已知大型犬种的幼犬分为一个对照组（每组4只动物）、三个实验组（每组4只动物）和一个长期组（6只动物）。对照组双侧腓骨干骺端插入不透射线的标记物，此外，一侧进行腓骨截骨术。在实验组中，进行腓骨转换，选择一根腓骨作为带血管移植物，另一根作为无血管移植物。使用系列X线片、组织学检查、荧光骨标记和微血管造影对对照组和实验组进行评估。术后1周、6周、3个月和7个月，每组动物处死。带血管的骺板移植和对照组均观察到持续生长，无统计学差异，而无血管移植物生长明显较少或无生长。带血管移植物平均长度增加21.2毫米，而无血管移植物增加6.6毫米。组织学检查、荧光染料骨标记和微血管造影证实，与无血管移植物相比，带血管的骺板移植具有持续的活力。
